The Comparative Research On Chemical Constituents Of Curcumae Radix, Curcumae Longae Rhizoma And Curcumae Rhizoma

Multi-origin Chinese herbal medicine, one herb originated from more than onespecies of plants, is a common phenomenon but important issue in traditional Chinesemedicine (TCM). Curcumae Longae Rhizoma, Curcumae Radix and CurcumaeRhizoma are such kind of multi-origin Chinese herbal medicines, originated fromCurcuma genus plants C. longa, C. wenyujin, C. kwangsiensis and C. phaeocaulis,respectively. The Curcuma plants are widely distributed in Sichuan, Zhejiang, Guangxiand Yunan Province of China. Curcumae Longae Rhizoma are the dry rhizomes of C.longa, Curcumae Rhizoma are the dry rhizomes of C. wenyujin, C. kwangsiensis and C.phaeocaulis, and Curcumae Radix are tuberous roots of those four plants. Thepharmacological activities of TCMs are correlated to their chemical compositions, sothe comparison of the chemical components of Curcumae Longae Rhizoma, CurcumaeRadix and Curcumae Rhizoma is helpful for their further pharmacological studies. Thisthesis is the comparative analyses of the secondary metabolites (curcuminoids andvolatile constituents) and primary metabolites (fatty acids) in those three TCMs. Thethesis includes four chapters.The first chapter is literature review, the research progress on chemical components,separation and analytical technique and pharmacological effects of Curcumae LongaeRhizoma, Curcumae Radix and Curcumae Rhizoma were summarized. Their chemicalcomponents are similar, they all mainly contain curcuminoids and volatile constituentsbut different in amount and composition. The volatile compounds were usually analyzedby gas chromatography (GC), and the curcuminoids were usually determined by highperformance liquid chromatography (HPLC) and capillary electrophoresis (CE). Thosethree TCMs all have the activities of anti-tumor, anti-bacterial and anti-oxidant, etc.In Chapter two, a HPLC method with double wavelength detection was developedto simultaneously determine secondary metabolites including curcuminoids (curcumin,demethoxycurcumin and bisdemethoxycurcumin) and volatile constituents (curdione,curdione, isocurcumenol, furanodienone, curcumol, germacrone and furanodiene) inthose three TCMs (24batches samples). The chemical (secondary metabolites)differences between Curcumae Radix and Curcumae Longae Rhizoma, Curcumae Radixand Curcumae Rhizoma, as well as within Curcumae Radix and Curcumae Rhizomawere compare, respectively.. The results showed that different herbs from multi-origins have significant difference in terms of content and components of curcuminoids andvolatile constituents.In Chapter three;gas chromatography–mass spectrometry (GC-MS) was used toanalyze fatty acids (primary metabolites) in those three TCMs. And the data wereanalyzed by multivariate statistics analysis methods of orthogonal partial least squaresdiscriminant analysis (OPLS-DA) and Hierarchical cluster analysis (HCA);. And theresult showed that the fatty acid patterns of those three TCMs are different;so they canbe clearly distinguished by primary metabolites (fatty acids).In conclusion;although all the three TCMs contain fatty acids;curcuminoids andvolatile compounds;the contents and some compositions are different in them.Therefore;those three TCMs should be carefully differentiated in the clinicalapplications.