| Objective: To investigate the effect of bone marrow mesenchymal stem cells in liveanimals hypertrophic scars.Discussion on feasibility of bone marrow mesenchymal stemcells for the treatment of hypertrophic scars.Methods:6Healthy adult male New Zealand rabbits.Designed Hypertrophic Scar of entrywhose diameter is6mm on rabbit auricular gaster side,each rabbit ear had6hypertrophicscars.Randomly selected6rabbits,the left ear is experiment group(a total of36wounds),the right ear is control group(a total of36wounds).Experiment group respectively,in the2nd day(20days after surgery) and the10th day(30days after surgery) after thecompletion of epithelialization,application of5th generation with BrdU labeled of BMSCswhich grew well and injected0.2ml to ring-shaped above cartilage layer of rabbit auricularhyperplastic scar.Control group injected the same dose of PBS buffer.The experimentalgroup and control group were based on the7day(37days after surgery)after second celltransplantation.The gross appearance of hypertrophic scar was recorded for eachexperimental group,Observation for the histology by HE stain and VG stain.Usedimmunofluorescence staining to observe the survival of stem cells in the experimentalgroup.Real time-Polymerase Chain Reaction(Real Time-PCR, RT-PCR) to detect mRNA ofDecorn(DCN) and transforming growth factor-β1(TGF-β1) in hypertrophic scar tissueexpression changes.Used Elisa to detect the DCN, TGF-β1, collagen I and collagen IIIprotein expression in hypertrophic scar tissue.Results:37days after the operation, scar hyperplasia was obvious in control group, the scarcolor was red, hard texture, extruded of the skin surface;the experimental group of scartissue volume reduced, scar tissue became flat and soft, color slightly lighter.HEstaining:basal cells of the control group arranged disorderly,it could be observed bymicroscope that a lot of fibroblasts proliferation and inflammatory cellsinfiltrated,fibroblasts ranged unordered, or arranged in spiral shapes;the basal cells of experimental group were arranged neatly, the number of fibroblasts near the superficiallayer of dermis was significantly reduced and neat,connective tissue hyperplasiareduce,only observed a small amount of inflammatory cell infiltration.VG staining:themicroscope observed in the control group there was a large number of collagen fibersdeposition,collagen fiber was bulky,arrangement out of order;the deposition of collagen inexperimental group decreased,the arrangement of collagen fibers not only loose andneat.Observed the cell survived in the tissue by immunofluorescence staining.RT-PCRresults:compared with the control group,the experimental group TGF-β1mRNA expressionwas significantly lower, DCNmRNA expression was significantly higher,the difference hadstatistical significance(P<0.01).Elisa results:compared with the control group,theexperimental group TGF-β1protein,Collagen I protein,Collagen III protein expression waslower,DCN protein expression was higher,the difference had statisticalsignificance(P<0.05).Conclusion:After the rabbit ear wound epithelialization complete, be transplantedBMSCs,can up-regulate the expression of DCN, down regulate the expression ofTGF-β1,reduce the synthesis of collagen I and collagen III, reduce the deposition ofextracellular matrix proteins, promote the orderly arrangement of the collagen,inhibithypertrophic scar effect. |
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