The Changes Of Intestinal Flora And Intestinal Mucosal Permeability In30Cases Of Children With Severe Hand-Foot-Mouth Disease

Objective: To observe the changes of intestinal microflora and mucosal barrierpermeability in30cases of children with severe Hand-Foot-Mouth Disease.Methods: The feces of30children with severe Hand-Foot-Mouth Disease were selectedas study group in Shenzhen Children’s Hospital from April to August2012and May toJuly2013, and32healthy children were selected as control group.The blood samples werecollected at the same time in the30children with severe Hand-Foot-Mouth Disease and30healthy children. The contents of bifidobacteria and E.coli in stool were detected byTaqman real-time PCR, the levels of Endotoxin、DAO and D-lactate in the serum weredetected respectively by improved chromomeric substrate azo, improved method ofo-dianisidine agent and enzymologist spectrophotometers.Results: The stool contents of bifidobacteria of patients in the early stage are lower thanthat in convalescent stage (8.80±0.61vs9.27±0.97, P<0.05) and show significantdifference,but they and healthy children are no significant difference (early:8.80±0.61vs9.28±0.89, P>0.05;convalescent:9.27±0.97vs9.28±0.89, P>0.05). The contents of E.coli of patients in the early stage and convalescent stage show no significant difference(9.21±0.82vs9.03±0.81, P>0.05), but they are significantly higher than that of healthychildren (early:9.21±0.82vs7.88±1.32, P <0.05; convalescent:9.03±0.81vs7.88±1.32, P <0.05).There are no significant difference of the value of B/E (Bifidobacterium/Escherichia coli) between the early stage and the convalescent stage (0.97±0.12vs1.03±0.20, P>0.05),but they are significantly lower than that of healthy children (early:0.970.12vs1.22±0.26, p<0.05; convalescent:1.03±0.20vs1.22±0.26, p<0.05),and thestatistical analysis is significant difference. There are no significant difference of levels ofendotoxin (0.50±0.31vs0.45±0.17, P>0.05); DAO (0.05±0.04vs0.05±0.03, P>0.05)and D-lactate (5.58±7.83vs3.66±8.05, p>0.05) between the patients and healthychildren. Conclusion: The intestinal microflora composition changes in children with severeHand-Foot-Mouth disease, In the early stage,the contents of bifidobacteria of patientswere lower，but the contents of E.coli of patients were higher. The value of B/E(Bifidobacterium/Escherichia coli) in the early stage and the convalescent stage weresignificantly lower than that of healthy children. There were no significantly changes ofintestinal mucosal barrier permeability in severe Hand-Foot-Mouth disease children.