A Preliminary Study Of LDHB Expression In Endometriosis And Its Clinical Significance

Objective: To examine the expression of LDHB in human endometrium and endometriosistissue.Method:1.20cases of endometriosis specimens were collected in our hospital fromApril2011to December2011that were treated with operation, including10cases ofadenomyosis and10cases of ovarian endometrial cyst.8cases of uterine myoma treatedwith uterine resection were collected as control group. Fresh normal endometrium andectopic lesions were collected and protected in liquid nitrogen immediately after operation.2. Total protein was extracted from the tissue samples. Western blot assay was used todetect the LDHB expression in the specimens and the semi-quantitative analysis wasperformed.3. The samples were embedded in paraffin, sliced, and immunohistochemistrywere used to detect the LDHB expression. Results: Compared with normal endometrium, the expression of LDHB in ovarianendometrial cyst was decreased with significant difference (P <0.01). Compared withadenomyosis, the expression of LDHB in ovarian endometrial cyst was decreased, andthere was significant differences (P<0.05). Compared the proliferative phase of normalendometrium with the secretory phase, there was no difference in the expression of LDHB(P=0.187).Conclusions: The expression of LDHB was significantly decreased in ovarian endometrialcyst. This finding might be related to the invasion and metastasis behavior of endometriosis.The LDHB expression in adenomyosis was higher than that of ovarian endometrial cyst,consistency with expression of normal endometrium, suggesting adenomyosis wasdependent on aerobic oxidation of glucose for energy as normal tissues.