Modulation Of The Expression Of Myocardial ATP-sensitive Potassium Channel By Remote Trauma And Ischemic Preconditioning

Objective:1. To investigate the expression of Kir6.2in cardiomyocytes after remote preconditioning of trauma (RPCT).2. To investigate the expression of sarcolemmal ATP-sensitive potassium channel in cardiomyocytes after remote ischemic preconditioning (RIPC).Methods:1. A rat model of abdominal surgical incision was employed and the expression of Kir6.2in cardiomyocyte was measured in control group and groups at15min,4h or24h postoperatively.2. Rat models of remote ischemic preconditioning were established by clipping bilateral femoral artery5minutes and opening5minutes for3cycles. Then, the expression of KATP channel in cardiomyocyte was evaluated in control group and groups at1h,4h,24h and48h postoperatively.Results:1. The relative protein expression level of Kir6.2in cardiomyocyte was increased after RPCT.2. The relative protein expression level of sarcolemmal ATP-sensitive potassium channel in cardiomyocytes was increased after RIPC.Conclusion:1. The expression of Kir6.2in cardiomyocytes is increased after RPCT, it may gives some hints for RPCT’s protection of heart against ischemic injury.2. The expression of sarcolemmal ATP-sensitive potassium channel was increased after RIPC, the change of expression in ATP-sensitive potassium channel may play a role in the cardiac protection of RIPC against ischemia.