Based On The Theory Of "Medicinal Guiding" Explore Musk Promote Exogenous RBMSCs With The Effect Of Migration And Its Mechanism

Objective: To study on effects of different doses of musk on migration ability ofexogenous rat bone marrow mesenchymal stem cells (BMSCs) in vivo of rat bonedefect models and observe the expression effect of it to monocyte chemoattractantprotein,mcp-1,and its receptor CCR2and stromal cell derived factor stromal cellderived factor-1,SDF-1,and its receptor CXCR4.Combine TCM medicinal guidingtheory with cytology,aiming at exploring relation between it and the theory of stemcell migration and to provide theoretical basis for migration mechanism anddemonstrating and explaining guiding theory,sessence from angle of modernmedicine.Methods: Using adherence screening method to culture BMSCs in vitro, identifyBMSCs through the observation of cell morphology, adipogenic and osteogenicinduction and cell phenotype identify. After rats Skull bone defect models were builtsuccessfully, replant BMSCs cultured in vitro.The model rats were randomly dividedinto four dose groups of high, medium, low and the blank control group. and gavagedmusk ketone according to respective dose, normal salinethe in blank control group.Once a day,their modeled skulls were taken out14days later,decalcified andsectioned.Observed expression of chemotactic factor in bone defect siteSDF-1,MCP-1, with method of immunohistochemical staining and change ofcorresponding chemotactic factor receptor,CXCR4,CCR2,with method of situhybridization.Observed, photographed in ordinary optical microscope.The4vieweach section were randomly select ed, the IOD value was calculated by IPP6.0software with the pictures,which was statistically analyzed.Results:The purified BMSCs adherent grow showing homogeneous,longfusiform fibroblast-like, eddy-like hover-arrangement. The expression of CD44、CD90were positive,while the expression of CD45were negative.The BMSCsdifferentiated to adipogenic and osteogenic after induced.Musk ketone can promoteexpression of chemokine factor, SDF-1、MCP-1in injured parts of skull defect ratmodel. There was significant statistical difference between the blank control group and different dose groups (P<0.01), in which the low group was the best(P<0.05).Musk ketone can promote expression of corresponding chemotactic factorreceptor, CXCR4、 CCR2, in injured parts of skull defect rat model. There wassignificant statistical difference between the blank control group and different dosegroups (P<0.01), in which the low group was the best (P<0.05).Conclusion:The mechanism of musk promoting exogenous BMSCs migrating toinjured parts in vivo of rats is close related to promoting expression of chemokinefactor and corresponding chemotactic factor receptor. Probably in this way, muskpromote rBMSCs migrate towards injured parts and thus develop its effect ofguiding theory.