Solanum Nigrum And Vascular Endothelial Growth Factor(VEGF) Antibodies On Colorectal Cancer Angiogenesis

Objective and BackgroundExperimental study of colorectal cancer chick transplantation model, and theexpression of Solanum nigrum ang VEGF antibody impact on angiogenesis of colorectalcancer. Compare adding Solanum nigrum and joining VEGF antibody differences chickchorioallantoic membrane, size and density of tumor angiogenesis; whileimmunohistochemical staining of vascular endothelial growth factor (VEGF) expression.Immunohistochemistry was used to detect CD34antibody (cell surface salivary mucin)expression derived tumor microvessel density and experimental applications LEICA imageacquisition processing and analysis software objectively reflect the situation regarding thedifferences chick angiogenesis and VEGF antibody then analyzed Solanum nigruminhibition of embryo transplantation model of colorectal cancer angiogenic growth of thismechanism.Solanum nigrum is an annual herb, the whole plant contains solanine, Australiasolanine, Australia eggplant side alkali and other alkaloids, earlier used in folk medicineas antitumor effects. This view of tumor angiogenesis by Folkman [1] in1971firstproposed that tumor growth is closely related to tumor angiogenesis. Use of the SolanaceaeSolanum genus, clinically also found a variety of solid tumors and leukemia have goodcontrol effect, at home and abroad in recent years for its anti-tumor effect and mechanismof action of the active ingredients began to get involved. Solanine can change the numberof multiple aspects of membrane protein function, membrane permeability and enzymeactivity, such as ATP to change the structure and function of the cell membrane, so as toachieve the anti-tumor effect. Solanum glycoproteins found on the HT-29(humancolorectal cancer), MCF-7(human breast cancer cells), HCT-116(human colorectalcancer cells), and other tumor cell cytotoxic and pro-apoptotic effects. Recent studieshave shown extracts of Solanum nigrum in promoting apoptosis of tumor cells to increaseintracellular synthesis, which may promote one of its mechanisms of apoptosis [2]unlimited proliferation of tumor cells with the characteristics of laser confocal microscopy (LCM) observed solanine can significantly reduce S180and H22tumor cells in mice RNA/DNA values, so that the formation of tumor cell DNA metabolism of RNA transcriptionis inhibited intracellular gene product-protein synthesis is blocked [3]. Some studiescomparing inhibition of the normal development of blood vessels, administered at15mgdose group when angiogenesis inhibition is not obvious, and when reached25mg Solanumnigrum, the embryo development was inhibited or killed, eight chick5embryo deathsoccur in the sample. When the dose of Solanum nigrum20mg, administered visibleembryo forming activity, on the next administration CAM support the emergence of a lackof blood vessel area after48h.Found Solanum nigrum extract can reduce the number ofU266cells in G0-Gl phase of the cell cytotoxicity observed Solanum nigrum extract onU266(myeloma cells), S, G2-M phase cells increased the number [4]. Solanum nigrumethanol extract can significantly prolong the survival time of tumor-bearing mice livercancer, inhibit the growth of sarcoma [5]. Solanum nigrum with detoxification, bloodswelling, diuresis Tonglin effect, the clinical use of Solanum nigrum decoction or injectiontreatment of cervical cancer, esophageal cancer, breast cancer, lung cancer, liver cancer,choriocarcinoma, ovarian cancer, sarcoma, etc. a variety of malignancies, have achieved acertain effect [6]. Solanum nigrum can inhibit chick chorioallantoic membraneangiogenesis [7]. The experimental results show that low-energy laser irradiation combinedSNL can inhibit VEGF production in tumor cells, leading to inhibition of angiogenesislinks, reduce tumor angiogenesis, tumor growth and blocking the necessary nutrients,inhibition of tumor cell growth, invasion, transfer [8]. However, Solanum nigrum whethercolorectal cancer angiogenesis inhibition effect, still no corresponding studies related to therole of endothelial cells has not been reported. Therefore, the design of the subject.MethodsOur study took9-day-old chick embryo as a model. Firstly, we transplanted human coloncarcinoma HT-29cells to chick embryo chorioallantoic membrane (CAM), and addedsolanine solution (experimental group1), VEGF antibody (experimental group2) andsterile PBS solution (control group) respectively to the sample which had cultured for oneday in incubator. After five days, we observed angiogenesis of tumor chick chorioallantoicmembrane, and measured density and area of the microvessel. Secondly, we took aphotograph by stereo microscope and archived it. Besides, we analyzed the capillary area of chick chorioallantoic membrane which respectively belonged to the experimental groupand the control group by taking advantage of Image proplus6.0software. Finally, using thetwo-step method for SP immunohistochemistry, we checked the microvessel densitymarked by CD34antigen of the tumor tissue on the chicken chorioallantoic membranetumor biopsy embedding in paraffin, and we made use of SPASS17.0software to analyzeand calculate the microvascular area of each group.ResultsThrough the analysis of experimental data, there were significantly difference of themicrovessel area and microvascular density by comparing the experimental group1and2with the control group, P<0.05, and it was significant in statistics. Besides, there also weresignificantly difference between the experimental group1to the experimental group2,P<0.05, it was significant in statistics as well.Conclusion:Solanine and VEGF antibodies can inhibit the growth of new blood vessels of HT-29tumorcell embryo, and the inhibited effect of VEGF antibody is superior to solanine.