The Research Of The Relationship Between Omentin, Adiponectin And Macroangiopathy In Type2Diabetes

ObjectiveTo explore the change of omentin and adiponectin in normal glucose regulationand type2diabetes complicated with macrovascular complications throughdetermining the serum levels of omentin, adiponectin and correlative metabolicindicators.To research the relationship between omentin and adiponectin,anddiscuss the relevance in omentin and risk factors of diabetic macrovascularcomplications. So as to explore the role the two adipokines play in the developmentof macroangiopathy in type2diabetes.MethodsAccording with require,88cases, who were outpatients, inpatients, or healthyphysical examines in the First Affiliated Hospital Of Nanchang University duringJuly2013to October2013,were recuited in this research.and they were divided into4groups: healthy control group including20cases(group NC), group AS including22normal glucose regulation patients with macrovascular complications, group DM andgroup DM+AS each group including23type2diabetes patients without and withmacrovascular complications.The data of these cases,for example gender, age, height,weight, blood pressure, duration of type2diabetes, were recorded and body massindex (BMI) was calculated. Fasting serum omentin and adiponectin levels weremeasured by enzyme-linked immuno sorbent assay (ELISA). The serum levels offasting blood glucose (FBG),2-hour postprandial blood glucose (2hPG), fastingplasma insulin (FINS), hemoglobinA1c (HbA1c), renal function, blood lipid,C-reaction protein were also tested. Meanwhile insulin resistance index wascalculated by the method of home ostasis model assessment: HOMA-IR=FBG×FINS/22.5. All data were statistically analyzed by t-test, ANOVA, Pearsoncorrelation analysis and multiple linear regression analysis.ResultsCompared with NC group, the DM and AS group serum omentin concentrationdecreased（P＜0.01）, DM+AS group serum omentin concentration decreased more significantly（P＜0.01）. NC group, AS group, DM group, DM+AS group,serumAPN levels of the four groups showed a gradual downward trend, and the differenceswere statistically significant (P <0.01or P <0.05). The CRP levels of DM group, ASgroup were significantly higher than NC group (P<0.01), the DM+AS group serumCRP levels were significantly higher than the DM group, AS group (P<0.01).Between male group and female group there were statistically significant differencesin the levels of serum omentin. Female group serum omentin concentration washigher than male group (P <0.05). Pearson correlation analysis showed that serumomentin concentration was positively correlated with HDL-C, APN（r=0.295、r=0.739）, and was negatively correlated with duration of type2diabetes, SBP, DBP,BMI, FBG,2hPG, HbA1c, HOMA-IR, CRP（r=-0.662、r=-0.344、r=-0.288、r=-0.551、r=-0.289、r=-0.434、r=-0.355、r=-0.282、r=-0.513）. Serum omentin concentrationhas no correlation with Age, FINS, TG, TC, LDL-C. Multiple linear stepwiseregression analysis showed that APN, BMI, duration of type2diabetes independentlyassociated with serum omentin(R2=0.546，R2=0.652，R2=0.689).Conclusions:1. Atherosclerotic or type2diabetic patients serum omentin and APN levelswere significantly lower than healthy people, with the extended duration of diabetesand the occurence of diabetic macrovascular disease, serum omentin and APN levelsdecreased more significantly. Meanwhile serum omentin concentration was positivelycorrelated with APN, suggesting that omentin, APN may be involved in thedevelopment of type2diabetes as well as its macrovascular complications.2. Serum omentin levels was negatively correlated with HOMA-IR, BMI, andwas positively correlated with APN, besides,BMI, APN is independent factors ofserum omentin levels, suggesting that obesity, obesity-related insulin resistance mayassociated with the generation of omentin. The regulation of omentin may depend onadiponectin.