Screening Anti-Radiation Bioactiv Ingredients In Si-Wu-Decoction Via TLR5-NF-κB Signal Pathway And Mechanismech Research

Radioprotectors are needed to protect cancer patient with radiotherapy and otner potentially exposed populations, such as workers in nuclear-power industry, space travelers or operators on radioactive devices. Traditional Chinese medicine has drawn increased attention because of its low toxicity. To screen bioactive ingredients in Si-wu-decoction rely on the TLR5-NF-κB signal pathway. Some researches focus on the molecular mechanisms of irradiation induced AHH-1cells damages and protective effects of TMP by means of cellular and molecular technologies. The conclusions are below:1. Fructose, adenine, folic acid, scopoletin, ligustrazine hydrochloride, β-Sitosterol could activate TLR5receptor-mediated NF-κB to some extent.2. Irradiation increased cell death, DNA fragmentation, activated Caspase activation and cytochrome c translocation, downregulated B-cell lymphoma2(Bcl-2) and upregulated Bcl-2-associated X protein (Bax). Pretreated with TMP significantly reversed this tendency. Several anti-apoptotic characteristics of TMP, including the ability to increase cell viability, inhibit Caspase-9activation, and upregulate Bcl-2and downregulate Bax in4Gy-irradiated lymphocytes were determined. Signal pathway analysis showed TMP could translate nuclear factor-κB (NF-κB) from cytosol into the nucleus.In a Conclusion, the results suggest that six anti-radiation bioactive ingredients in Si-wu-decoction via TLR5-NF-κB signal pathway are found. TMP had a radioprotective effect through the NF-κB pathway to inhibit apoptosis, and it may be an effective candidate for treating radiation diseases associated with cell apoptosis.