Detection Of Epidermal Growth Factor Receptor Gene Mutation And Clinicopathological Characteristics Of Patients With Non-small Cell Lung Cancer In Chongqing Municipality

Objective: To investigate the mutation status of epidermal growthfactor receptor gene and Clinicopathological characteristics of patients withnon-small cell lung cancer in Chongqing Municipality.Methods: Clinical specimens from55NSCLC patients were analyzedfor EGFR mutations in exons18,19,20and21by usingamplificationrefractory mutation system(ARMS)following genomic DNAextraction from paraffin-embedded tissue or malignant pleural effusion.Results: sensitive EGFR mutations were detected in14patients among55patients (25.45%). The mutation rate in male and femalewas12.50%,43.48%respectively (P=0.009). Smoking, non-smokingpatients mutation rate was11.11%and39.29%respectively (P=0.017).Squamous carcinoma and non-squamous cell carcinoma(adenocarcinoma，Adenosquamous carcinoma) mutation rate was0.00%and33.33%(P=0.041). Mutation in malignant pleural effusion(MPE) and Formalin Fixed Paraffin Embedded(FFPE) was33.33%and22.50%(P=0.636). Mutation rate in exon19,21was57.14%(8/14),35.71%(5/14) respectively; only1specimen was detectedEGFR mutation both in exon18and20(7.15%,1/14). EGFR mutation ratein exon19in patients under and over the age of60years old was100%，28.57%respectively(P=0.021).Conclusions: Our results demonstrated EGFR mutation rate weresignificantly higher in female，non-smoking and non-squamous cellcarcinoma (adenocarcinoma and Adenosquamous carcinoma) NSCLCpatients from Chongqing Municipality. EGFR mutations in MPE and FFEPwere similar. The most common EGFR mutations were deletions in exon19and the L858R substitution in exon21. EGFR mutation in exon19and21was associatied with the age of patients.