Correlation Between Inducible Nitric Oxide Synthase Gene Polymorphism And Affectability Of Kawasaki Disease Coincidenced Coronary Artery Lesion

Objective: To investigate the correlation between inducible nitric oxidesynthase (iNOS) gene polymorphism and the development of coronaryartery lesion(CAL) in Kawasaki disease(KD).Methods: Totally146KD patients as KD group and119healthychildren as control group were included from Children’s Hospital ofChongqing Medical University. There were79KD patients with CAL asCAL group and67patients without CAL as NCAL group. The SNPs ofiNOS gene loci-277T/C,-1026C/A and+2087G/A were genotyped by usingMassARRAY-IPLEX technology and matrix-assisted laser desorptionionization time of flight inass spectrometry platform (MALDI-TOF-MS).Relation between gene polymorphisms and the development of CAL in KDwas analysed. The plasma iNOS level was determined by ELISA, the plasmaNO level was measured by nitric acid reductase, and expression levels wereboth compared between groups.Results:①The plasma iNOS level was significantly lower in control group（48.02±31.46）U/L than that in KD group（86.45±44.25）U/L（P<0.05）,however, There were no significant difference between CAL group(90.29±47.68）U/L and NCAL group（81.46±41.32）U/L （P>0.05）.②The plasma NO level were no significant differences between control group（55.09±33.68）μmol/Land KD group（50.09±32.56）μmol/L (P>0.05),however, it was significantly higher in CAL group(58.10±40.45）μmol/Lthan that in NCAL group （39.69±14.04）μmol/L（P<0.05）.③As for the-277T/C locus of iNOS gene, there were no significant differences betweenthe frequeneies of genotype and allele of KD and control groups or CAL andNCAL groups(P>0.05).②For the-1026C/A locus of iNOS gene, there wereno significant differences between the frequeneies of genotype and allele ofKD and control groups or CAL and NCAL groups (P>0.05).③For the+2087G/A locus of iNOS gene, there were no significant differencesbetween the frequeneies of genotype and allele of KD and control groups;However, there were significant differences between CAL and NCALgroups（P<0.05）.④The frequencies of haplotypes combined by-277T/Cand-1026C/A were no significant differences between KD and controlgroups or CAL and NCAL groups (P>0.05).⑤The frequencies ofhaplotypes combined by-1026C/A and+2087G/A were no significantdifferences between KD and control groups (P>0.05); However, Thefrequencies of haplotype-1026C/+2087A were different between CAL andNCAL groups（P<0.05）. The-1026C/+2087A haplotype was associated with a significantly decreased risk of CAL[OR (95%CI)=0.45(0.22～0.91),P<0.05].Conclusion: iNOS gene+2087G/A polymorphism and-1026C/+2087Ahaplotype are associated with the development of CAL in KD,+2087A allelemay significantly reduce the risk of CAL by lowering plasma iNOS level toreduce NO production. Plasma iNOS and NO are important in thedevelopment of vasculitis and CAL in KD.