Effects Of XiaoBanXiaTang On Guinea Pig Ileum Contractile Activity In Vitro

Objective: To observe the contractility of isolated ileum of guinea pig, andanalyze the mechanism of anti-emetic effects of XiaoBanXiaTang (XBXT). Method:Using emesis-relating agonists Acetylcholine(ACh), Histamine(His),5-hydroxytryptamine(5-HT), Dopamine(DA) as tool drugs, to observe the effects ofXBXT, Ginger juice and Pinellia alkaloids on contraction of isolated ileum. Results:XBXT, Ginger juice and Pinellia alkaloids could dose-dependently inhibit isolatedileum spontaneous contractions, reducing the average tone, amplitude and frequencyof contractions. ACh, His and5-HT stimulated contractions of the guinea pig ileum,enhanced the tone, frequency and amplitude. Pretreating with above three tool drugsrespectively, then herbal solution was added into organ bath, or reversed the treatment,all XBXT and Ginger juice and Pinellia alkaloids could suppress the contractility,significantly reduced the average tone, amplitude, frequency and maximal tone ofileum contractions stimulated by above three tool drugs. DA inhibited thespontaneous contraction, reduced the tone, frequency and amplitude. Pretreating withDA, then herbal solution, or reversed the treatment, all XBXT and Ginger juice andPinellia alkaloids could antagonise the inhibitory effect of DA, enhanced the averagetone, amplitude, frequency of the isolated ileum stimulated by DA to some extent.Conclusion: XBXT, Ginger juice and Pinellia alkaloids could inhibit guinea pigileum spontaneous contractions, the mechanism underlying their inhibiting effectsmight be blocking M receptor, H1receptor,5-HT receptor and D2receptor, orsuppress the ileum smooth muscle directly. The further mechanisms of the anti-emeticeffect of XBXT need to be explored.