| Purpose/Objective:This study aimed to investigate the expression changes of SUMO-1gene in rathepatocellular carcinoma model and further explored the role of SUMO-1in livercarcinogenesis.Methods:70male rats were randomly divided into experimental model group（n=50）andcontrol group（n=20）. The model was established by diethylnitrosamine (DENA)-induced hepatocarcinogenesis. The rats in experimental model group were drunk bywater with95ug/ml DENA for30days, sterile water for30days and then water with95ug/ml DENA to the end. The rats in control group were drunk by normal water allthe time. All the rats were fed by general diet. The liver tissues were taken for3ratsin the experimental model group every2weeks from the2nd week(2,4,6,8,10,12,14,16,18,20w). The liver tissues of2rats in control group were taken every2weeks.Pathology of liver cancer model were observed using pathological section. TheSUMO-1gene expression in mRNA and protein level was tested by RT-PCR andwestern blotting respectively.Results:The hepatocellular carcinoma model induced by DENA from inflammatory liverinjury to the liver cancer were established succeessfully. The SUMO-1geneexpression in mRNA and protein level was generally increased during the formationof hepatocellular carcinoma model. By contrast, the expression of SUMO-1in controlgroup was lower and not changed over the time.Conclusion:The expressions of SUMO-1at mRNA and protein level of were graduallyincreased during the formation of hepatocellular carcinoma. It may play a key role inthe formation of hepatocellular carcinoma. |
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