A Study Of The Expression Of LSD1in Renal Clear Cell Carcinoma

Objective：This study is mainly focused on the association between renal clear cellcarcinoma and Lysine specific demethylase1(LSD1) by using RT-PCR andimmunohistochemical method.Methods：1．All patients in this study were received an Laparoscopic radical nephrectomy（LRN） surgery in department of Urology of First Affiliated Hospital of NanchangUniversity from July2012to December2012. Total of30renal cell carcinomapatients, the30tissue adjacent to carcinoma and normal renal tissues as controls wereconfirmed by pathology.18cases of30patients with renal cell carcinoma in men andwomen in12cases,30cases of renal cell carcinoma patients had complete clinicaldata. All cases were confirmed by operation and pathology. Preoperative withoutradiotherapy, chemotherapy, targeted therapy of drugs and immune therapy, etc. Thegrouping of Fuhrman classification method according to the WHO in1997recommended: high differentiated group and6cases of differentiation group (19cases),5cases poorly differentiated group2．To analyze the significance of the expression of LSD1gene in renal clear cellcarcinoma, the tissue adjacent to carcinoma and normal kidney tissues, by usingRT-PCR.3．To analyze the significance of the expression of LSD1protein in renal clearcell carcinoma, the tissue adjacent to carcinoma and normal kidney tissues, by usingimmunohistochemical method.4．The frequency distribution of each group was calculated by direct countingmethod. The statistical analysis were conducted by SPSS13.0. For the distributions ofpopulation gene frequency, Chi-square test is used. Relative risk was described by theodds ratio (OR) and95%confidence interval (95%CI). To evaluate the associationsbetween renal cell carcinoma and LSD1through case-control study. Result：1. There were statistical significances in the distribution between renal cellcarcinoma and adjacent to carcinoma groups(χ2=13.125，P＜0.01) using RT-PCR.Compared to the tissues adjacent to carcinoma groups, LSD1gene increased the riskto renal cell carcinoma with OR of7.667(95％CI was2.424~24.245).2. There were statistical significances in the distribution between renal cellcarcinoma and normal renal tissues groups(χ2=19.288，P＜0.01) using RT-PCR.Compared to normal renal tissues groups, LSD1gene increased the risk to renal cellcarcinoma with OR of13.143(95％CI was3.837~45.023).3. There were not statistical significances in the distribution between the tissuesadjacent to carcinoma groups and normal renal tissues groups(χ2=0.800，P＞0.05)using RT-PCR.4. There were statistical significances in the distribution between renal cellcarcinoma and adjacent to carcinoma groups(χ2=27.149， P＜0.01) usingimmunohistochemical method. Compared to the tissues adjacent to carcinoma groups,LSD1protein increased the risk to renal cell carcinoma with OR of29.571(95％CIwas6.851~127.637).5. There were statistical significances in the distribution between renal cellcarcinoma and normal renal tissues groups(χ2=32.411， P＜0.01) usingimmunohistochemical method. Compared to normal renal tissues groups, LSD1protein increased the risk to renal cell carcinoma with OR of45.000(95％CI was9.732~208.077).6. There were not statistical significances in the distribution between the tissuesadjacent to carcinoma groups and normal renal tissues groups(χ2=0.417，P＞0.05)using immunohistochemical method.Conclusion：LSDl high expression was detected in renal clear cell carcinoma, so we canpresume that LSD1may exist correlation with the incidence of renal carcinoma,what’s more, LSDl may become the new therapeutic targets for renal clear cellcarcinoma.