| Objective: To explore the value of candidate miRNAs in tumorigenesis andprogression of renal cell carcinoma through detecting the expression of candidatemiRNAs in renal clear cell carcinoma tissues and corresponding adjacent tissue andanalyzing its relationship with clinical pathological features。Methods:20fresh renal clear cell carcinoma tissue samples were collected fromhospitalized patients of Department of Urology in First Affiliated Hospital ofNanchang University from September2009to January2011after resection of kidneyand kept in liquid nitrogen. The expression levels of miRNA-20b, miRNA-21,miRNA-34a, miRNA-142-5p miRNA-210, miRNA-141, miRNA-200c, miRNA-200b, miRNA-514and miRNA-532-5p in renal clear cell carcinoma tissues andcorresponding adjacent tissue were analyzed by RFQ-PCR.2-△△CTmethod was usedto analyze the experimental data. The experimental data were expressed as mean±standard deviation. The experimental results were analyzed by paired samples t-testand two-sample t test in SPSS17.0statistical software.P value less than0.05wasconsidered statically significant.Results: miRNA-142-5p were not amplificationed in cancer tissue andcorresponding adjacent tissue.Compared with adjacent tissue, miRNA-21、miRNA-34aand miRNA-210were high expressed in renal cell carcinoma while miRNA-141andmiRNA-200c were low-expressed(P＜0.05), the expression levels of miRNA-20b,miRNA-200b, miRNA-514and miRNA-532-5p kept unchanged. The expression levelsof miRNA-34a increased with the tumor grade. Conclusions: miRNA-21, miRNA-34a, miRNA-210, miRNA-141and miRNA-200c were differential expressed in renal clear cell carcinoma; they may be usedas potential diagnostic and therapeutic targets for renal cell carcinoma. Objective: To explore the possible role of Differential express miRNA intumorigenesis and progression of renal cell carcinoma through its target genes.Methods: the target genes of Differential express miRNA were pedicted bytargetScan （http://www.targetscan.org）、 PicTar （http://picTar.mdc-berlin.de）、miRanda （http://microrna.sanger.ac.uk） miRDB(http://miRNAdb.org/miRDB),andtheir functions were analyzed in renal cell carcinoma with genbank database.Results: Target genes forecast analysis results show that the target genesfunction of Differential express miRNAs were very wide, involved in cellcycle,signal transduction, cell differentiation, cell proliferation, cell apoptosis, andother aspects. A total of11target genes with related renal cell carcinoma werepredicted: miRNA-21(4)，miRNA-34a(8)，miRNA-210（1），miRNA-141（4），miRNA-200c(6).Among of them,TGFBR1, ITGB3, VHL, VEGFA, GSTM3andCYP1B1is pedicted for two or more miRNA.Conclusion: Bioinformatics analysis shows that the difference of theexpression miRNA may play an important role in tumorigenesis and progression ofrenal cell carcinoma by its target genes. Objective: To explore the value of miRNA-34a、 Notch1and Hes1intumorigenesis and progression of renal cell carcinoma through detecting theexpression of miRNA-34a, Notch1and Hes1in renal clear cell carcinoma tissuesand corresponding adjacent tissue and analyzing its relationship with clinical path-ological features.Methods:48fresh renal clear cell carcinoma tissue samples were collectedfrom hospitalized patients of Department of Urology in First Affiliated Hospital ofNanchang University from September2009to January2011after resection of kidneyand kept in liquid nitrogen. The expression levels of miRNA-34a、Notch1and Hes1in renal clear cell carcinoma tissues and corresponding adjacent tissue were analyzedby RFQ-PCR;2-△△CTmethod was used to analyze the experimental data. Theexperimental data were expressed as mean±standard deviation. The experimentalresults were analyzed by paired samples t-test and two-sample t test and itsrelationship between clinical pathological features were analyzed by spearmancorrelation analysis in SPSS17.0statistical software. P value less than0.05wasconsidered statically significant.Results: In48cases of renal cell carcinoma specimens，miRNA-34a expressedhighly in33cases,8cases of low expression and7cases of nondiscrimination.Notch1expressed lowly in36cases,3cases of high expression and9cases ofnon-discrimination. Hes1expressed lowly in31cases, nine cases of highexpression, eight cases of non-discrimination. The expression of miRNA-34a、Notch1and Hes1in renal cell carcinoma were times more than those in the adjacentnormal tissue,4.093±3.661;0.497±.724;0.498±.872respectively.The resultswere statistically significant (P<0.05). The expression of miRNA-34a was positivelycorrelated with pathological grade,while Notch1was negatively(P<0.05).Theexpression of miRNA-34a and Notch1was correlated with age,.miRNA-34a waspositively correlated with less than50years,while Notch1was negatively(P <0.05).But both of two had no different in sex, tumor size and clinical stage of cases.The expression of Hes1related to clinicopathological parameters was notstatistically significant (P>0.05).Conclusion: The expression of miRNA-34a、Notch1and Hes1was different inrenal clear cell carcinoma.The miRNA-34a and its target genes Notch1may beinvolved in the progression of renal clear cell carcinoma.It may be a potentialdiagnostic and therapeutic target for renal cell carcinoma. |
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