A Study On P16Gene Mutation In Pterygium

Objective：To investigate the role of p16gene mutation in pterygium. Discussion themutation of the exon1and exon2of the p16gene in the pterygium and its effect onpterygium pathogenesis.Methods：Forty cases of pterygium and conjunctiva tissues were collected. DNA wasextracted from pterygium tissues and conjunctiva tissues by DNA extract Kits, exon1and2of p16gene of40patients with pterygium and40normal controls wereamplified by polymerase chain reaction(PCR), and mutation of p16gene (exon1and2) in all subjects were screened by PCR-single strand conformation polymorphism(SSCP). Further sequencing was performed in samples with abnormal SSCP results,in order to confirm the mutation and its location.Results：In forty cases of pterygium, the frequency of p16genic point mutation were22.5%(9/40), respectively, were significantly higher than in the conjunctiva group. Inwitch, the mutation of p16gene exon2was obviously higher than in the p16geneexon1(P<0.05). The mutation of p16is correlated with occupation (P<0.05)，but nodistinct difference with clinical parameters such as sex, age, clinicopathologic stageand recurrence (P>0.05)．By sequencing, nine cases of pterygium tissue, relativelynormal, all has changed. Such as: exon1(P6), the sequence of76base pairs, Greplaced by the T,exon2b(P32), in sequence of624base pairs, T replacement into A,exon2c(P14), in sequence of644base pairs, G replacement into A and so on.Conclusion：1.High mutation rates of p16gene existed in pterygium. It demonstrated that themutation of p16might play an important role in the pathogenesis of pterygium.2. The mutation of p16gene exon2was visibly higher than in the p16geneexon1.Implying that p16gene exon2may play an important role in pterygium. 3. Detected p16gene mutation and its correlation with the patient’s occupationprompted the development of the potential role of the p16gene in pterygium, p16gene mutation mechanism is not clear, maybe is related to the role of ultravioletradiation.