Study Of The Colony Forming And Methylation Of P16gene Promotor In Bone Marrow-derived HPP-CFC Of Psoriatic Patients

Objective:To investigate the activity of bone marrow–derived hematopoietic cells of psoriatic patients and the relationship between the methylation status of P16 gene promoterMethods:Bone marrow-derived mononuclear cells were separated by density gradient centrifugation. the bone marrow mononuclear cells were cultured.in methycellulose semi-solid culture medium with SCF,GM-CSF,IL-3 and IL-6 for 14 days, and then high–proliferative potential colony–forming cells(HPP-CFC) were counted.The HPP-CFC were collected and its genomic DNA was isolated . Methylation status of P16 gene promoter of DNA by sodium bisulfite was studied by the methylation-specific polymerase chain reaction (MSP).Results:In methycellulose semi-solid culture system ,the number of HPP-CFC in bone marrow of psoriatic patients was significantly less than that of normal control and diameter. The positive frequency of methylation of P16 gene promoter in HPP-CFC of normal contrasts was higher than that of psoriatic patients.Conclusions:The activity of of bone marrow–derived hematopoietic cells of psoriatic patients is abnomal. the lower positive frequency of methyllation of P16 gene promoter in HPP-CFC perhaps play a role in lower colony-forming capability of HPP-CFC of psoriatic patients.