Effect Of Low-dose X-ray Radiation On Adaptive Response In Gastric Cancer Cell

Objective:To study the effect and mechanism of low-dose radiation (LDR) on adaptive response of gastric cancer cell.Methods:SGC7901cells were cultured in vitro, and divided into four groups:control group (DO group), low-dose radiation group (D1group,75mGy), high-dose radiation group (D2group,2Gy), low-dose plus high-dose radiation group(Dl+D2group,75mGy+2Gy, the interval of low and high-dose radiation being8hours.) Cell inhibition rate was detected by CCK8method; the proportion of cell cycle at different times after irradiation was determined by using a flow cytometry. The ATM mRNA levels were detected by using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR).Results:There was no significant different between group DO and D1, groups D2and D1+D2in cell inhibition rate(P>0.05); There was a significant increase G2/M arrest in group D2and D1+D2than group DO and D1after6h of radiation and did not recover at48h(P<0.05); The ATM mRNA expression of group D2and D1+D2increased highly than that of group DO and D1(P<0.05); However, differences between group D2and D1+D2, group DO and Dl were not statistical significant(P>0.05).Conclusion:LDR cannot induce adaptive response in SGC7901cells in vitro, which may be associated the regulation of cell cycle, and its ATM mRNA expression cannot be affected by75mGy X-ray radiation.