The Expression And Clinical Significance Of FOXC2and FOXE1in Primary Hepatocellular Carcinoma

Objective:To study the expression of mRNA and protein of FOXC2and FOXE1in PHCC, and discuss the relations between the expression of mRNA of FOXC2/FOXE1and clinicopathologic indicatos.Methods:23cases of PHCC and the corresponding liver tissue just around the tumor (LAT) and5cases of normal liver tissues were collected. All the samples were detected by real-time fluorescent quantitative PCR and Western-blot to analysis the expression of FOXC2and FOXE1, then, immunohistochemisty was used to detect the location of FOXC2and FOXE1. At the same time, the relationships between the expression of FOXC2/FOXE1and clinicopathologic indicators were analyzed.Result1. Real-time Fluorescent quantitative PCR demonstrated that the expression of mRNA of FOXC2in PHCC was significantly higher than that in LAT and normal liver tissues(2.4650±0.9604vs1.1625±0.9317vs1.0244±0.9246respectively, P<0.05) and the expression of mRNA of FOXE1in PHCC was significantly lower than that in LAT and normal liver tissues (0.3881±0.1104vs0.7514±0.1567vs1.1794±0.1627respec-tively, P<0.05).2. Western-blot demonstrated that the expression of protein of FOXC2in PHCC was significantly higher than that in LAT and normal liver tissues (0.6357±0.3155vs0.0614±0.3031vs0.3031±0.0432respec-tively, P<0.05) and the expression of protein of FOXE1in PHCC was lower than that in LAT and normal liver tissues (0.2923±0.0503vs0.6710±0.0685vs0.7603±0.1116respectively, P<0.05)3. Overexpression of FOXC2revealed by immunohistochemical was predominantly localized in the cytoplasm of PHCC, meanwhile, lower expression of FOXE1revealed by immunohistochemical was predominantly localized in the nuclei of PHCC.4. The expression of FOXC2significantly rose in the higher Edmondson stage and the higher TNM stage, also in vascular invasion group and capsule invasion group(P<0.05), meanwhile, the expression of FOXE1significantly reduced in the higher Edmondson stageand the higher TNM stage, also in vascular invasion group and capsule invasion group (P<0.05), the expression of FOXE1was also significantly reduced inthe noncapsule group (P<0.05)Conclutions1. Both FOXC2mRNA and protein expressed were significantly upregulated in PHCC. The overexpression of FOXC2was significantly associated with Edmondson stage, TNM stage, vascular invasion and capsule invasion. It may play an important role in the development and progress of PHCC.2. Both FOXE1mRNA and protein expressed were significantly downregulated in PHCC. The lower expression of FOXE1was signifycantly associated with Edmondson stage, TNM stage, vascular invasion and capsule invasion, meanwhile, the expression of FOXE1was significantly reduced in the noncapsule group, FOXE1may play an important role in the development and progress of PHCC.