Determination Of Expression Levels Of VEGF-mRNA In Cervical Cancer Tissues By Real-time Fluorescent Quantitative RT-PCR

Objective To investigate the expression of vascular endothelial growth factor (VEGF) mRNA in cervical cancer tissues. The relationships between related mRNAs expression and the tumor metastasis, prognosis were analyzed.Methods The levels of VEGF-mRNA expression in 48 cases of invasive carcinoma of cervix, 30 cases of carcinoma in situ and 36 cases of normal controls were detected by TaqMan real-time fluorescent quantitative RT-PCR and analyzed in a conjunction with the clinical features of the tumor.Results There was a significant different levels of VEGF-mRNA expression between the tissues of tumor, carcinoma in situ and that of the normal controls. The levels of VEGF-mRNA expression in invasive carcinoma of cervix was higher than that in all matched. And there was no significant relationship between the levels of VEGF-mRNA expression with the pathological types in invasive carcinoma of cervix. But the levels of VEGF-mRNA expression was significantly associated with the clinical stage ,the grade of tumor pathology, lymph node metastasis, tumor size and the invasion of deep muscular layer (p<0.05).Conclusion The method of detection for the VEGF-mRNA expression in cervical cancer tissues by real-time fluorescent quantitative RT-PCR has a high sensitivity and specificity. It is reliable and easily performed. The results suggest that the VEGF may play an important role in genesis and development of invasive carcinoma of cervix. It could be a useful biological marker to foresee the lymph node metastasis and monitor the progression of invasive carcinoma of cervix. VEGF might be used as a potential molecular target for the treatment of malignant tumors.