| ObjectiveDNA methylation status of microRNA-375and its association withclinicopathologic features in gastrointestinal carcinoma and matched adjactnon-carcerous tissues were detected; meanwhile, the expression of3-phosphoinositide dependent protein kinase-1in gastrointestinal cacncer andnormal gastric mucosa was detected, the association between it and theclinicopathologic features in gastrointestinal cancer aslo were analyzed. Thenthe relationship between miRNA-375DNA methylation and PDK1ingastrointestinal cancer was investigated, in order to reveal the pathogenesis ofgastrointestinal cancer and to provide theoretical basis for early diagnosis ofclinical treatment.MethodsEighty cases of intestinal carcinoma and matched adjacentnon-cancerous tissues and forty cases of gastric carcinoma and matchedadjacent non-cancerous tissues were detected by DNA methylation specificpolymerase chain reaction(MSP), Immunohistochemistry SP method wasapplied to detect the expression of PDK1in gastrointestinal carcinoma. Finally,Spearman rank correlation analysis was performed to analyze the relationshipbetween miRNA-375DNA methlation and PDK1in gastrointestinal cancer.Results1. The positive rate of DNA methylation of microRNA-375gene promoter in colorectal cancer and adjacent non-cancerous tissues was60%(2440) and10%(440) respectively.There was stastistically significant difference betweentwo groups(P＜0.05). No significant association was found between themethylation rate of microRNA-375and the status of gender, age, Generalclassification, histological type, TNM staging system, lymphatic metastasis anddistant metastasis, CEA; The positive rate of PDK1expression in colorectalcancer and adjacent non-cancerous tissues was85%(3440) and25%(1040)respectively.There was statistically significant difference between twogroups(P＜0.05). No significant association was found between themethylation rate of microRNA-375and the status of gender, age, Generalclassification, histological type, TNM staging system, lymphatic metastasis anddistant metastasis, CEA; There was a significantly positive relationshipbetween the methylation of miRNA-375DNA promoter and the expressionintensity of PDK1(r=0.313,P＜0.05).2.The positive rate of DNA methylation of miR-375gene promoter ingastric cancer and adjacent non-cancerous tissues was80%(1620) and25%(520) respectively.There was stastistically significant difference betweentwo groups(P＜0.05). No significant association was found between themethylation rate of microRNA-375and the status of age, gender, Borrmanntype, TNM staging system, lymphatic metastasis and distant metastasis, CEA;The positive rate of PDK1expression in gastric cancer and adjacentnon-cancerous tissues was75%(1520) and30%(620) respectively.There wasstatistically significant difference between two groups(P＜0.05). No significantassociation was found between the methylation rate of microRNA-375and thestatus of age, gender, Borrmann type,TNM staging system, lymphaticmetastasis and distant metastasis, CEA; There was a significantly positiverelationship between the methylation of miR-375DNA promoter and theexpression intensity of PDK1（r=0.687,P＜0.05）. ConclusionsThe methylation of miR-375DNA promoter may occur in gastrointestinalcancer. Methylation of miR-375was uncorrelated with clinicopathologicfeatures; The expression of PDK1was upregulated in gastrointestinal cancer,the rate of expression was uncorrelated with clinicopathologic features; Thepositive relationship between the methylation of miRNA-375DNA promoterand the expression intensity of PDK1reveals that miR-375may take part in thedevelopment of gastrointestinal cancer by targeting PDK1. |
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