The Expression And Clinical Significance Of MiRNA-31?miRNA-105?miRNA-196b In Plasma Of Patients With Colorectal Cancer

Objective:To detect the expression of miRNA-31,miRNA-105 and miRNA-196b in plasma of patients with colorectal cancer(CRC)and to investigate the correlation of their expression and evaluate the relations of these microRNAs with clinical stage,lymph node metastasis and so on.Method:Plasma samples were obtained from 20 patients with CRC,10 patients with colorectal polyps and 10 healthy individuals for the present study.The plasma expression of miRNA-31,miRNA-105 and miRNA-196b in plasma samples of the three cohorts was detected using reverse transcription-quantitative polymerase chain reaction.The diagnostic value in the plasma of the patients with CRC was evaluated by receiver operating characteristic(ROC)curve.Result:1.The expressions of miRNA-31 and miRNA-196b in plasma of normal population,patients with CRC and colorectal polyps were no statistically significant difference(P>0.05).2.The expression of miRNA-105 in plasma of patients with CRC was significantly higher compared with the normal population and colorectal polyps,and there was statistically significant(P<0.05).3.The expression of miRNA-105 has no correlation with age,sex,the clinical stage and lymph node metastasis(P>0.05).4.ROC curve analysis demonstrated that,comparing patients with CRC with healthy individuals,the area under the curve of plasma miRNA-105 was 0.758,and its specificity and sensitivity were 60 and 90%(P=0.005).Conclusion:the results of the present study demonstrated that plasma miR-105 is upregulated in CRC,and may have an application as a diagnostic biomarker for patients with CRC.