Protective Effects Of Osthole On Intestinal Ischemia-reperfusion Injury In Mice

Objective: The aim of the present study was to investigate Osthole effect on intestinalischemia-reperfusion injury in mice.Methods: Mice model of intestinal I/R injury was established by clamping the superiormesenteric artery for120minutes and then relieving for60minutes. Forty-five maleKunming mice weighing27-31g were randomly divided into three groups. They aresham group(S group), model group (I/R group) and the osthole (Ost+group). After thetreatment, intestinal wet/dry weight ratios, superoxide dismutase (SOD),malondialdehyde (MDA) in serum were examined by colourimetric assay and diamineoxidase (DAO) was examined by automatic biochemical analyzer assay. The levels ofinflammatory cytokine (TNF-α, IL-1β, IL-2) were examined by enzyme-linkedimmunosorbent assay (ELISA). Other forty-five male Kunming mice weighing27-31gwere grouped and treated as above for Evans blue (EB) test.Results:1. Compared with the sham group, wet to dry weight ratios, Evans blue content andChiu’s score in intestinal tissues of mice were significantly increased in the modelgroup (P<0.05or P<0.01). Compared with the model group, wet to dry weight ratios,Evans blue content and Chiu’s score in intestinal tissues of mice were significantlylower in Ost+group (P<0.05or P<0.01).2. Compared with the sham group, the levels of TNF-α, IL-1, DAO and MDA in serum were significantly increased in model group (P<0.01or P<0.05). Compared with themodel group, the levels of TNF-α, IL-1, DAO and MDA in serum in Ost+group weresignificantly lower than those in the model group (P<0.01or P<0.05).3. Compared with the sham group, SOD activity and IL-2levels in serum weresignificantly increased in the model group (P<0.01). Compared with the model group,SOD activity and IL-2levels in serum were significantly increased in Ost+group(P<0.01).Conclusion:1. After ischemia reperfusion, the small intestine in mice was damaged obviously.2. Osthole exerted protective effect on intestine injury after ischemia reperfusion inmice.3. The protective effect may be inhibition of oxidative stress by directly attenuating thetissue damage or regulating the inflammatory associated cytokine to attenuate theischemia-reperfusion injury indirectly.