Expression And Significance Of Reactive Oxygen Species Modulator1in Human Rectal Cancer

Objective To investigate the expression of reactive oxygen speciesmodulator1（ROMO1） and explore its possible relationship withclinicopathologic feature of human rectal cancer.Methods Real-time quantitative polymerase chain reaction(RT-qPCR),immunohistochemistry and Western blot were used to detect the expressionof ROMO1and flow cytometry with fluorescence probe techniques wereused to determine the level of endogenous reactive oxygen species(ROS) in53cases of rectal carcinoma tissues and corresponding para-carcinomanormal mucosal tissues. The correlations of ROMO1to ROS andclinicopathologic features of rectal cancer was analyzed.Results The expression of ROMO1mRNA in rectal carcinoma tissuesis significantly higher than that in para-carcinoma normal musosal tissues(P＜0.05). The positive expression of ROMO1, which is localized in thecytoplasm, in rectal carcinoma(73.6%) is significantly higher than that inpara-carcinoma normal musosal tissues(54.7%)(P＜0.05). The higher thepositive rate of preoperative serum CEA, the more advanced Dukes staging, the higher the expression of ROMO1mRNA(P<0.05), and the larger thetumor size, the lower the degree of differentiation, the higher the positiverate of lymph node metastasis, the higher the expression of ROMO1mRNAand protein (P<0.05). The expression of ROMO1mRNA and protein wassignificantly positive correlation (r=0.431, P＜0.001). The level of reactiveoxygen species in rectal carcinoma tissues is significantly higher than that inpara-carcinoma normal mucosa tissues. The expression of ROMO1proteinwas significantly positively assocaiated with the level of ROS in rectalcancer tissue(r=0.628, P=0.009).Conclusion The high expression of ROMO1induced by the generationof reactive oxygen species play an important role in the tumorigenesis anddevelopment of the rectal cancer. ROMO1involves in malignant biologicalbehaviors of the tumor, such as cell proliferation, differentiation andmetastasis, sometimes might become used for predicting the occurrence andprognosis of rectal cancer. It might reveal the roles and pathway of ROMO1and explore new antineoplastic therapy targets to provide a new theory basis.