Experimental Research Of Bone Morphogenetic Proteins-2Transfection For Periodontal Bone Deficiency Treatments In VIVO

Background:Periodontitis is one of the most important diseases affecting humans’ health. The periodontal tissue destroyed especially the bone defect caused by periodontitis is hard to repair by common treatments. Recent years, a few periodontal regeneration treatments had been used in clinic such as guided tissues regeneration (GTR), application of cellar factors (CF). But the utilization was limited due to the deficiency of regeneration periodontal tissue and the curative effect unstable of the application of CF. The development of gene therapy provide new path to the treatment of periodontitis. Transfection of the bone morphogenetic proteins-2(BMP2) to target tissues can enhance the expression of CF, promote the precursor cells’differentiation, and finally enhance the formation of periodontal bone tissues.Objective:Construct the eukaryotic express vector pEGFP-N1-BMP2which contains the CDS fragment of BMP2.Transfect the vector into the SD rats with the deficiency of periodontal bones in vivo by partly injection and observe the regeneration of the periodontal bones after transfection.Methods:The total RNA was isolated from the chorion cells of human placenta and RT-PCR was employed to gain the CDS of human bone morphogenetic proteins-2gene. The CDS of BMP2gene was cloned into pMD19-T vector and transformed into the E.coil. Sequencing further validated the sequence of BMP2CDS. And then the correct CDS of BMP2was constructed into the pEGFP-N1vector to gain the transfectional vector pEGFP-N1-BMP2. The SD Rats which were lack of the periodontal bones by partly deboning were used as the animal model to transfect the pEGFP-N1-BMP2into the periodontal tissues using partly injection. And Observed the regeneration of periodontal bones after4weeks.Result:The pEGFP-N1-BMP2vector was constructed correctly. After4weeks, the experimental group(EG) could form more new alveolar bone, cementum and peridental membrane while the control group(CG) form less new alveolar bone and no cementum. The areas of the new alveolar bone was measured by image analyze software and the result showed that the EG was larger than CG (p<0.05). Meanwhile, the ratio of the heights of new alveolar bone was measured, and the data showed that the EG was also larger than CG (p<0.05).Conclusion:The transfection of the pEGFP-N1-BMP2into the SD rats which were lack of periodontal bones in vivo could enhance the regeneration of bone tissues. As the result of it, the BMP2might take part in the regeneration of the periodontal bones and this result might provide experiment basis for the treatments of bone deficiency caused by periodontitis.