Effect Of The Guanine Nucleotide Exchange Factor C3G Overexpression On The Survival Of Cardiomyocytes And Its Underlying Mechanisms

Objectives To observe the effects of the guanine nucleotide exchangefactor C3G overexpression on the survival of cardiomyocytes and itsunderlying mechanisms.Methods H9C2cardiomyocytes were transiently transfected withpCXN2-Flag and pCXN2-Flag-hC3G (overexpression of human C3GmRNA) plasmids, then treated with hypoxia/reoxygenation (H/R)respectively. The cardiomyocytes were divided into blank group, emptyplasmid group, C3G overexpression group, blank+H/R group, emptyplasmid+H/R group and C3G overexpression+H/R group. The expressionsof the C3G mRNA&protein and ERK1/2protein, and the apoptosis andproliferation rate were analyzed in the H9C2cardiomyocytes respectively.Results Compared with blank&empty plasimd groups and blank+H/R&empty plasimd+H/R groups, human C3G mRNA&protein, p-ERK1protein and proliferation rate were increased significantly, and apoptosis rate was decreased significantly in the C3G overexpression and C3Goverexpression+H/R groups respectively. Compared with blank+H/R andempty plasimd+H/R groups, p-ERK2protein was increased significantly inthe C3G overexpression+H/R group.(All P <0.05).Conclusions C3G overexpression can increase the ERK1/2proteinexpression, cell proliferation&survival and apoptosis inhibition in theH9C2cardiomyocytes; C3G overexpression also can decrease theH/R-induced inhibition of ERK1/2protein expression and cell proliferation&survival, and apoptosis in the H9C2cardiomyocytes. C3G overexpressioncan promote cell survival in the cardiomyocytes, it’s pro-survival effect maybe mediated by the increase of p-ERK1/2protein expression and apoptosisinhibition.