Effects Of Propofol On PI3K/AKT Pathway In Rat Lung Injury After Liver Ischemia Reperfusion

AIM: Through the study of propofol on phosphatidylinositol-3kinase protein serine/threonine kinase (PI3K/AKT) signal pathway in themodel of rat lung injury after hepatic ischemia reperfusion to discuss themechanisms of propofol in the lung injury after hepatic ischemiareperfusion.METHODS:66SD rats, randomly divided into4groups， shamoperation group (group S,n=6), IR group (group IR, n=24), propofol group(group P, n=24), propofol+Wortmannin group (group P+W, n=12)., IRgroup and P group according to the time of1h,3h,6h,12h afterreperfusion is divided into four subgroups, P+W group according to thetime3h,6h after reperfusion was divided into two subgroups. S groupwas only dissected porta without ligation; IR group was dealt with theligation of hepatic pedicle to make the liver ischemia30min and thenreperfusion for building the model of rat liver ischemia reperfusion. In Pgroup, rats with slow injection of propofol with20mg kg-1from the caudalvein10min before ischemia, and then was continuously pumped propofolwith20mg kg-1h-1until its death, remaining the same as IR group. The P +W group was injected the blocker of PI3K Wortmannin15g kg-1before ischemia, remaining as P group. Each group in reperfusion1h,3h,6h,12h in rat lung tissue death take. The Western blotting detection in ratlung tissue total Akt (t-Akt), phosphorylation Akt (p-Akt) and Bcl-2protein expression level; With Annexin-v FITC/PI method to detect lungcell apoptosis rate.RESULTS: Compared with S group, IR group, P group and P+Wgroup in the lung tissue p-Akt and Bcl-2protein expression level andlung cell apoptosis rate increased (P <0.05); And the IR groupcomparison, P p-Akt and Bcl-2protein expression levels, lung cellapoptosis rate lower (P <0.05), P+W group the index expressiondifference was not statistically significant (P>0.05); Compared with P, P+W group p-Akt and Bcl-2protein expression down, lung cellapoptosis rate increased (P <0.05); Show t-Akt protein expressiondifference was not statistically significant (P>0.05).CONCLUSION: Propofol can reduce rat liver ischemia-reperfusioninduced lung injury and its mechanism may and PI3K/Akt signalingpathways leading to the activation and then cut lung cell apoptosis related.