| Introduction:Study the endogenous hydrogen sulfide expressed in the the castrated rat corpus cavernosum to explore the mechanisms of erectile dysfunction in androgen deficiency.Methods:8-week-old healthy male SD rats (N=30) were randomly divided into six groups:two weeks of sham-operated group (A),4weeks of the sham group (B),2weeks castrated group (C),4weeks castrated4group (D), after castration androgen hormone replacement therapy2weeks group (E) and4weeks group(F). Rats in Experimental group were taken the surgical removal of both testicles, in control group were take sham-operated. Group E and group F were subjected to the physiological dose of testosterone propionate (TP)3mg/kg. day subcutaneous injected after castration, while the other group was given the isodose oil. The Concentration of serum testosterone, intracavernous pressure (ICP), mean carotid arterial pressure (MAP) of rats were detect two and four weeks Postoperative. Determine the concentration of H2S and mean carotid cystathionine-β-synthase (CBS), the protein content of the cystathionine-γ-lyase (CSE) expressed in the penis specimens by immunohistochemistry and Western blot.Results:Serum testosterone levels-in group C was significantly lower than group A, E(P<0.05), groups D was significantly lower than group B, F(P<0.05). As to5,7V electrical stimulation, ICP/MAP, H2S concentration levels, The CBS、 CSE protein expression amount were in the same situation. Compared to group D CBS、 CSE protein expression of Group C were significantly decreased (P <0.05).Conclusion:Expression of penile corpus cavernosum tissue CBS/CSE decreased caused by hydrogen sulf ide pathway inhibition may be one of the mechanisms of androgen deficiency caused by decreased erectile function of rats. |
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