The Effect Of BMSCs Transplantation Combined With Electro-acupuncture On The JNK Signaling Pathway And Local Blood Flow Regulation Factor Of Rats Subjected To Intracerebral Hemorrhage

Objective:Bone marrow mesenchymal stem cells (ipads marrow mesenchymal stem cells, BMSCs) transplantation combined with cupping (company-acupuncture, Ea) treatment of bleeding in the brain (Intracerebral hemorrhage, ICH) in rats, observed in brain tissue around hemorrhage stove and phosphorylation C-Jun amino terminal kinase (Phospho-C-Jun N-terminal kinases, p-JNK) silk crack the original activated protein kinase kinase4(MEK4) and silk crack the original7(MEK7) activated protein kinase kinase expression changes, detected blood endothelin-1(ET-1)and calcitonin gene related peptide (CGEP) content, explore the BMSCs transplantation combined with electroacupuncture of ICH after JNK signaling pathway and the influence of local blood flow adjustment factor. Methods:-80cryopreservation of BMSCs recovery training batches, for cell growth around80%fusion, will culture fluid replacement for induced liquid cultivation of24hours in advance, then change for6h without serum induced liquid induced digestive cells, adjust cell concentration is2.5×107/ml standby by brain stereotaxic apparatus by using collagenase and heparin into the caudate nucleus preparation of SD rat ICH model in2h,24h,48h after time of SD rats nerve function Score (Neurological Severity Score, NSS), a Score greater than or equal to9points in experiment and BMSCs Ea-BMSCs group randomly divided into Control group and Ea group four,3days after surgery by brain stereotaxic apparatus, the original channel will be20μl0.9%saline and20μl BMSCs suspension moving into ICH caudate nucleus of rats in each group respectively according to treatment time of rats were divided into Id,3d,5d,7d,14d five subgroups electric acupuncture group and Ea-BMSCs group will take the da-zhui acupuncture point and best point for curative treatment, once a day until the execution groups of rats in the corresponding time points draw blood and beheaded in brain, using protein immunoblot (Wetern Blotting, WB) method to detect p-JNK, MEK4and MEK7expression level, radioimmunity analytic method to determine the content of ET1and CGRP in the blood. Results:(1) the induced BMSCs training6h, under a microscope observation of BMSCs into round or oval, refractive sexual enhancement, nucleus cells surrounded by one or several long protrusions, show neuron model change, raised a woven mesh with each other;(2) each group p-JNK expression gradually increased, peak5days, after gradually decreases; The same point in time, BMSCs Ea-BMSCs group Ea group protein expression were higher than in the Control group, difference has statistical significance;(3) KEK4and MEK7protein expression in the BMSCs group Ea-BMSCs were higher than in the Control group and Ea group, and respectively in the5d and7d peak time of Ea-BMSCs group protein expression significantly increased relative to the Control group compared with the Control group with significant difference (P<0.05).Ea-protein expression significantly increased relative to the Control group, BMSCs group was statistically significant, P<0.05; Pure electric acupuncture group in1d MEK4and MEK7protein expression and occurrence peak, compared with the Control group with significant difference P<0.05;(4) the BMSCs Ea-BMSCs group expression of ET-1Ea group were higher than Controla group, P<0.05, there is statistical significance; BMSCs Ea group in addition to the1d expression of ET-1below Ea-BMSCs group, P<0.05;(5) in addition to the1d, BMSCs group, Ea-BMSCs group expressed CGRP Ea group were higher than in the Control group, P<0.05, with statistical meaning. Conclusion:1. Morph-ological observation of BMSc culture show a neuron model change.2. Electro-acupuncture and BMSCs transplantation joint application, a simple BMSCs transplantation can activates JNK signaling pathway, which is beneficial to inhibit apoptosis of injured neurons to protect neurons.3.Compared with BMSCs transplantation, combined application of electroacupuncture and BMSCs transplantation can increase the expression of ET-1and CGRP, and Promote the blood vessel function recovery after intracerebral hemorrhage...