Expression Of Toll-like Receptor4and Impact Of MiR-TLR4Interference On HBV-related Hepatoma Cell

Purpose:To investigate the effects of toll-like receptor4on gene expression and cellgrowth in human HBV-related hepatocellular carcinoma cells.Methods:Toll-like receptor4protein expression was analyzed in five hepatoma cell lines(Hep3B, HepG2.2.15, HepG2, SMMC7721and Huh7) using western blotting. Thecell with TLR4overexpression was selected to be further researched. Four groups(normal group, negtive control group, plasmid no.3group and plasmid no.4group)were divided. After interfering the TLR4expression in overexpression hepatoma cellvia miR-TLR4plasimids, cell proliferation was measured by colorimetric methylthiazolyl tetrazolium (MTT) assay; cell clonogenicity was detected by cloneformation assay; and cell apoptosis and cycle distribution were examined by flowcytometry.Results:TLR4was experssed in all the cell lines, and it was higher in Hep3B followedby SMMC7721、HepG2.2.15、HepG2and Huh7. Compared with the control groups,interfering the expression of TLR4could cause cell cycle block at G2/M phase andS phase(P＜0.05), decrease the percentage of cells at G1phase(P＜0.05), inhibit theproliferation and cloning efficiency of Hep3B(P＜0.05), and promote apoptosis(P＜0.05).Conclusion:The expression of TLR4is up-regulation in HBV-related hepatocellularcarcinoma cells. Interfering the expression of TLR4in vitro have influence on Hep3Bcell growth and cell cycle, including inhibiting the proliferation, cloning efficiency ofHep3B, blocking cell cycle at G2/M phase and promoting apoptosis.