Pathological Change Of Experimental Sputum Stasis Model And The Study On The Effect Of The Intervention Of Traditional Chinese Medicine

Research purpose:Hide sputum and hide stasis theory is that the face of coronary heartdisease,sputum and stasis Hidden in the body, condenses into virus,hidden in the blood. Evil can never prevail over good, hidden in thebody bided and hair; In the acute phase, it is very evil and easy totouches and hair; In the stationary phase, it can overcome evil, evilhide in bided and hair. Because of Hide sputum and hide stasis don’tget long and thorough intervention, so the cardiovascular event chainrepeated sequential happen. Based on the above views, This topic throughthe establishment of “V evil of sputum and stasis” coronary heartdisease animal model,and through the observe of atherosclerotic plaqueformation before and after to observe the regular changes of the lawof blood biochemical and vascular pathological,and observe theinfluence of the traditional chinese medicine shen hong hua zhuo tongluo ke li. To provide the experiments evidence for coronary heart diseaseof V evil of sputum and stasis, And form paper.Research methods:1、Divided the experimental rats into four groups:blank group、model group(use high fat forage and intraperitoneal injection of vitaminD3), The medium dosage of medicine group(Feeding high fat forage andintraperitoneal injection of vitamin D3and Lavage12g/kg of traditionalChinese medicine shen hong hua zhuo tong luo ke li) and High dosetreatment group(Feeding high fat forage and intraperitoneal injectionof vitamin D3and Lavage24g/kg of traditional Chinese medicine shenhong hua zhuo tong luo ke li). According to the weight proportion, blankgroup and model group lavage with normal salineIn addition to blank group, The rest of groups one-time intraperitoneal injection of VD3600000IU/kgFeeding after four weeks,and For testing samples after eight weeks.2、With urethane20%intraperitoneal injection of the anesthesia,abdominal aorta in blood taking and serum separating for blood fat test.3、Use enzyme linked immune method to test NO、ET、TXA2、PGI2、TNF-α、MMP-9、ICAM-1、VCAM-1.4、Separate thoracic aorta and abdominalaorta,HE dyeing with sudan Ⅲ.5、In dose and high dose of medicine shenhong hua zhuo tong luo ke li to intervene.6、Evaluation of traditionalChinese medicine shen hong hua zhuo tong luo ke li on the influence ofthe above indicators.Research results:1、TC、TG、LDL-C、HDL-C:①model group、the dosage and high dosegroup compare with blank group, TC、LDL-C、HDL-C are significantlyincreased(p<0.01);TG no obvious rise.②The dosage of medicine groupcompare with model group, TC rise(p>0.05); TG no obvious rise(p>0.05);LDL-C rise(p>0.05); HDL-C rise(p>0.05).③high dose group compare withmodel group, TG have no obvious rise(p>0.05);TC have obviousrise(p<0.05); LDL-C rise(p>0.05); HDL-C obvious rise(p<0.05).④Highdose group compare with the dose,TC have no obvious decrease(p>0.05);LDL-C have no obvious decrease(p>0.05); HDL-C have no obviousrise(p>0.05);TG have no obvious decrease(p>0.05).2、Thoracic aorta HE staining，Blank group intima smooth, no creamchange, Model group intima smooth, with a little fat deposition, no creamcolor change, The dosage and high dose treatment group intima smooth,did not see the cream color change, four groups structure basic normal,did not see the obvious change.Conclusion1、High fat feed feeding can make rat TC、LDL-C and HDL-C increasedsignificantly, forming high cholesterol.2、High fat feed with intraperitoneal injection of vitamin D3, failure to form a atheromatousplaque.3、AS experimental research shows, Take the dose lavage of shenhong hua zhuo tong luo ke li to reduce rat blood TC、TG and LDL-C effectis not obvious, to increase high density lipoprotein has certaincurative effect. Rats with shen hong hua zhuo tong luo ke li lavage didnot see obvious intervention effect.