The Experimental Studies On Expression Of ILK Andα-SMA In Different Periods Of Liver Damage And On Anti-Liver Fibrosis Of Irbesartan

Objectives:To explore the mechanism of Irbesartan’s anti-fibrosis by detecting the expression of ILK and α-SMA in different periods of liver damage, and to further search the new way of treatment of liver fibrosis.Methods:After free actibity for a week,10rats selected randomly from all of the80healthy male Sprague-Dawley rats were killed,whose liver tissues were as normal control specimens.The others were divided into3groups:liver fibrosis grouop(30rats),Irbesartan group(30rats) and the control group(10rats).The liver fibrosis group were received subcutaneous injection of CCL4twice a week,so as to the Irbesartan group which was administered Irbesartan by gavage,the control group received subcutaneous injection of Olive oil.At the second week,the fourth week,the sixth week and the eighth week,we respectively killed6rats from the liver fibrosis group and the Irbesartan group,taking the liver specimens which were quick-frozen in liquid nitrogenand firstly into-70℃refrigertor.The control group was administered as before at the eighth week.We could assess the degree of liver fibrosis by HE staining,detect the ILK and α-SMA by real-time PCR and immunohistochemical staining.Results:At the eighth week,the liver fibrosis group has2rat with liver cirrhosis and4rats with obvious liver fibrosis;all of the rats from the Irbesartan group have obvious liver fibrosis;the control group rats have no liver fibrosis.The dates of HE staining suggest that the degree of liver fibrosis becomes more and more serious with prolongation of experiment time at the liver fibrosis group and the Irbesartan group,and that the fibrosis of Irbesartan group is ligther than the fibrosis of liver fibrosis group,the dates have statistical significance(P<0.05).At the liver fibrosis group,the expression of ILK and α-SMA is significantly increased in contrast to the control group,and the expression is gradually increased in 2,4,6,8weeks liver tissues,the dates have statistical significance(P<0.05).The expression of ILK and α-SMA at the Irbesartan group is less than that of at the liver fibrosis group,but is more than the expression of ILK and α-SMA at the control group,the dates have statistical significance(P<0.05).At liver fibrosis group and the Irbesartan group,the expression positions of ILK and α-SMA in liver is almost same, the positions mainly include hepatic perisinusoidal space of Disse, portal area of fiber proliferation and near the fiber menbrane.Conclusion:As CCL4acting on liver,the expression of ILK and α-SMA is gradually inceased,the degree of the liver fibrosis becomes more and more serious.Irbesartan can significantly alleviate hepatic fibrosis progress which may be associated with Irbesartan’s inhibition the expression of ILK.ILK could be as a potential detection indicators used in the detection of liver fibrosis, and as a potential therapeutic target for the treatment of liver fibrosis.8diagrams,5tables,38references.