Effect And Mechanism Of Liver Fibrosis In Rats HSYA A Induced By Carbon Tetrachloride

Objective To investigate the effects and mechanism of hydroxysafflor yellow A on hepatic fibrosis caused by carbon tetrachloride in rats.Methods Thirty-six SD rats were randomly divided into three groups:model group, HYSA group and control group, each group consists of12animals. The models were induced by carbon tetrachloride, combined with alcohol and high fat diet. The model group and HYSA group were treated with saline and HYSA respectively for8weeks. Rats of the control group were given soybean oil by intragastric administration for9weeks. The hepatic tissues of rats in the three groups were taken at the end of the ninth week. To determine the pathological changes and grades of hepatic fibrosis, the obtained slides of liver tissue were stained by hematoxylin and eosin (HE), and Masson. The expression of TGF-β1and CTGF were detected by means of immunohistochemistry staining.Results①HE staining:the structure of the acini hepatic disordered in rats of the model group, there were pseudolobuli and fibrous tissue hyperplasia, which were more severe in the portal area. Numerous hepatic cells were of ballooning degeneration, adipose degeneration and many cells necrosis. For rats in the HYSA group, the structure of the hepatic lobule was almost normal. The connective tissue between the lobules hyperplasia, there were cellular edema and adipose degeneration, but the phenomenon was slighter compared with that of the model group. The structure of the hepatic lobule, hepatic cords and hepatic sinusoid were normal in rats of the control group.②Masson staining:There were collagen fibers hyperplasia and fibers around the hepatic lobules, the structure of the hepatic lobules destroyed and many pseudolobuli formed in rats of the model group. The collagen fibers around the hepatic lobules also hyperplasia in rats of the HYSA group, but there was no pseudolobuli. For rats in the control group, the structure of the hepatic lobule was normal, with few collagen fibers surrounding the lobules and portal areas.③The collagen fiber deposit area of the hepatic tissue and the semiquantitative analysis of fibrosis showed that there were severe fibrous degeneration in rats of the model group than that of the control group (P<0.05),while these two index were not so high in the HYSA group, compared with that of the model group (P<0.05)④For rats in the model group, the TGF-β1mostly expressed in the portal areas and the hyperplastic collagen fibers, which was higher than that of the control group (P<0.05), the levels of TGF-β1were lower in RAPA group than that in the model group (P<0.05).⑤The CTGF was expressed in the cytoplasm, which was higher in rats of the model group (P<0.05), the positive cells were mostly hepatic cells and the stellate cell of liver, also there were positive cells in the portal areas. Compared with the control group, the levels of CTGF were lower in RAPA group, with positive hepatic cells and cells located in the portal areas (P<0.05)Conclusions①Hepatic fibrosis was successfully established by administering carbon tetrachloride, combined with alcohol and high fat diet.②HYSA could reduced hepatic fibrosis caused by the substances mentioned above.③HYSA reduce the degree of hepatic fibrosis through inhibiting TGF-β1and CTGF expression in the hepatic tissues.