Effects Of Swimming Training On Behavioristics And Synaptic Plasticity Of Middle Cerebral Arterial Occlusion Rats

ObjectivesStroke is a leading cause of disability in today’s society. The purpose of rehabilitation after stroke is to improve function after stroke and to reduce long-term disability. In order to provide experimental basis for cerebral infarction rehabilitation, we discuss the effects of swimming training on behavioristics and synaptic plasticity with Middle Cerebral Arterial Occlusion rats.MethodsWe set up MCAO model by male Sprague Dawley rats. MCAO rats were forced to execute swimming training, then we recorded their neural function defect score and front and hind legs grasping function score. Finally, we calculate the volume of cerebral infarction by2,3,5-Triphenyltetrazolium chloride staining, the number of apoptotic cell of ischemia half dark domain by Nissl’s staining, and we detected the degree of activation of Caspase-3,8,9on peripheral tissue of cerebral infarction by spectrophotometry. We also measured brain slices’Potential changes of Long-term Potentiation(LTP) and Long-term Depression(LTD) by brain slice patch clamp recording.At the same time, we observed the expression of neural particles by using Real-time PCR technique.ResultsWe set up MCAO rats’ swimming training model successfully and found that after the rats had underwent swimming training13days, neural function defect score, the volume of cerebral infarction, the degree of activation of Caspase-3,8,9all decreased and the survival rate of cell of ischemia half dark domain increased(P<0.05). Patch clamp recording from brain slices of the MCAO rats showed that LTP of striatum and hippocampal slices strengthened through swimming training(P<0.05). Real-time PCR results suggested the expression of neural particles significant increased(P<0.05).ConclusionsThrough swimming training, the recovery of neural function after cerebral infarction may be enhanced, the degree of cerebral infarction may be reduced and the synaptic plasticity may be improved, the changing of synaptic plasticity may be associated with increased expression of neural particles.