Research On Cell Proliferation And Apoptosis Of Osteosarcoma Cells MG63Transfected By RASSF1A Gene

Objective:RASSF1A was a new candidate tumor suppressor genes,which could play a role of tumor suppressor in most of the tumors. This subject was to further discusse RASSF1A gene transplantation for cell proliferation and apoptosis of osteosarcoma cells MG63.To further confirm the role of suppressor and mechanism in the tumor. It could provide basis of experimental and theoretical for the new gene replacement therapy using in the clinic field.Materials and methods:1. Bulid PCDNA3.1(+)-RASSF1A and identify it, The plasmids inclinding pCDNA3.1(+)-RASSF1A and pCDNA3.1(+) were separately transfered into osteosarcoma cells MG63by Liposome transmission method.2. Western-blot was used to detect the RASSF1A protein expression separately in pCDNA3.1(+)-RASSF1A> pCDNA3.1(+) and osteosarcoma cells MG63group.3. MTT method was separately used to dedect cell proliferation in three groups at24h,48h and72h after RASSF1A Genetic transmission.4. TUNEL method was separately used to dedect cell proliferation in three groups at24h,48h and72h after RASSF1A Genetic transmission.The experimental results:1. RASSF1A gene could be transfected successfully to osteosarcoma cells MG63and pCDNA3.1(+)-RASSF1A was the first time displyed in the field of osteosarcoma cells.2. RASSF1A protein expression could be seen in pCDNA3.1(+)-RASSF1A group,but the other two groups had no protein expression,which proved protein expression missing occured in osteosarcoma cells MG63.3. The growth of osteosarcoma cells MG63was inhibited obviously after RASSF1A gene transfered when compared with the other two groups(p<0.05).4. The apoptotic index was increased when compared with the other two groups after RASSF1A gene transfered(p<0.05).Conclusion: RASSF1A gene could be transfected successfully to osteosarcoma cells MG63, and RASSF1A protein expression missing in osteosarcoma cells. RASSF1A could play a role of tumor suppressor which molecular mechanism maybe by cell proliferation inhibition and cell apoptosis facilitation.