The Expression And Significance Of Wnt5a And Twist In Benign And Malignant Breast Tissues

Objective:The experiment will research on breast lobular hyperplasia, breast precancerous lesions, breast carcinoma in situ and invasive breast carcinoma and discuss the affect of Wnt5a and Twist during the development of breast cancer at the level of protein. It preliminarily clarify the relationship between Wnt5a and Twist. All of these will provide the basis theory to molecular targeted therapy of breast cancer.Methods:Using the immunohistochemical staining method to detect the expression of Wnt5a and Twist in various kinds of breast disease tissues. Using the SPSS software to analyze the relationship between these two markers and clinicopathological parameters of patients and analyze the relativity of these two protein.Results:1. The positive staining of the Wnt5a in cytoplasm and cell membrane was seen, mainly in cytoplasm; The positive expression rate of the Wnt5a in breast lesion tissues:breast lobular hyperplasia was75.00%, breast precancerous lesions was62.50%, breast carcinoma in situ was44.44%, invasive ductal carcinoma was20.83%and invasive lobular carcinoma was31.25%(5/16);compared with the non-cancer group (69.44%), the positive expression rate of wnt5a in breast cancer group (28.57%) showed a trend of significantly reducing, P=0.000;From breast lobular hyperplasia and breast precancerous lesions to carcinoma in situ, especially to invasive carcinoma, the positive expression rate of Wnt5a showed a trend of gradually decreasing, P=0.001.2. The positive staining of the Twist in cytoplasm and nucleus was seen, mainly in cytoplasm; The positive expression rate of the Twist in breast lesion tissues:breast lobular hyperplasia was25.00%, breast precancerous lesions was37.50%, breast carcinoma in situ was55.56%, invasive ductal carcinoma was70.83%and invasive lobular carcinoma was68.75%; compared with the non-cancer group (30.56%), the positive expression rate of Twist in breast cancer group (67.35%) showed a trend of significantly increases, P=0.001; From breast lobular hyperplasia and breast precancerous lesions to carcinoma in situ, especially to invasive carcinoma, the positive expression rate of Twist showed a trend of gradually increasing, P=0.005.3. Spearman correlation analysis showed that the positive expression of Wnt5a and Twist is negatively correlated in various stages of breast tissues (rs=-0.387, P=0.000).4. The expression of Wnt5a and Twist were unrelated with age, menstrual status, tumor location and size, clinical stage and Her-2expression of patients. The expression of Wnt5a and ER is positively correlated (rs=0.366, P=0.010). And the expression of Wnt5a and PR is also positively correlated (rs=0.411, P=0.002). But There is no correlation between the expression of Twist and ER (P>0.05), so do the expression of Twist and PR.Conclusion:1. The expression of Wnt5a in the multi-stage breast lesions respectively shows trend of gradually decreasing. This suggests that the expression of Wnt5a is negatively correlated with the degree of malignancy of breast cancer.2. The expression of Twist in the multi-stage breast lesions respectively shows trend of gradually increases. This suggests that the expression of Twist is positively correlated with the degree of malignancy of breast cancer.3. The expression of Wnt5a and Twist is negatively correlated in various stages of breast tissues. This suggests that Wnt5a signaling pathway play a significant role in the initiation and progression of breast cancer by interacting with the Twist signaling pathway.4. The expression of Wnt5a is positively correlated with the expression of ER and PR. This might suggests that Wnt5a may acts as a new marker which can predict the sensitivity of endocrine therapy and the clinical prognosis. We also could speculate that Wnt5a may be a new therapeutic target to regulate the expression of ER\PR of breast cancer.