Effects Of Sodium Cyanide Poisoning And Hypothermal Exposure On Heart, Liver, Renal Of Rabit

Objective：Cyanide is a chemical warfare agent of foreign key equipment, can be used for avariety of environmental battlefield. But there is a certain toxic effects of differentbattlefield environment for cyanide. Our previous study has confirmed that, in high altitudehypoxic environment, increase the toxicity of cyanide to the experimental animal, theclinical manifestations, treatment methods and normal environment difference of cyanidepoisoning. The cold is a common special environment. Future possibility of cyanide in localwar operational use in the cold environment of the great. The project is on the basis ofprevious work, using NaCN as the representative to cyanide poison, artificial lowtemperature freezer simulation in cold environment, built environment of cyanide poisoninganimal model, in the heart, liver, kidney function as observation indexes, changes of heart,liver, kidney function animal under different temperature environment cyanide poisoning,preliminary study on low temperature exposure of cyanide poisoning effect on animal bodyfunction. To investigate the therapeutic effect of4-DMAP in the treatment of lowtemperature environment of cyanide poisoning and on this basis. The development of thework, for the special environment of cyanide poisoning to add new knowledge, but alsoprovide an important basis for the treatment of cyanide poisoning in special environment,has important significance for improving our army chemical weapons attack ability underlow temperature environment.Methods：1. Artificial low temperature freezer simulation. In cold environment, a lowtemperature environment of cyanide poisoning animal model. Low temperature test cabinet by the laboratory freezer with Haier restructuring electronic temperature controller and acontrol group,20℃,2℃,-20℃applications such as temperature, temperature error is lessthan0.5℃.2. Japanese white rabbits as experimental objects, were randomly divided into6groups, namely,20℃,2℃,2℃,-20℃,+NaCN,-20℃+NaCN,2℃+NaCN+4-DMAPgroup,5rabbits in each group. Animal Anesthesia after normal operation, carotid artery intubation,establish the blood channel,60min adapted to the experimental environment. For theexperimental group animal intraperitoneal injection of NaCN2mg/kg, the treatment groupanimal by intraperitoneal injection of sodium cyanide (2.0mg/kg) immediately after,intramuscular injection of4-DMAP (3.2mg/kg). In zeroth,30,60,90,120min point intime from the left carotid arterial blood5mL, centrifugal separation of blood plasma (4℃,2000r/min,10min, centrifugal), ultra low temperature freezer-80℃frozen.3. By HITACHI company production of Hitachi7020automatic biochemical analyzer,kit for use in Sichuan Mike bio technology company production of determination ofmyocardial enzymes, liver, kidney function and biochemical indexes, biochemical indexesto observe the changes of heart, liver, kidney function in each group of experimentalanimal.Results：1. The temperature decrease, myocardial enzymes CK, CK-MB, LDH, α-HBDH indexchanges, and exposure time in the same direction and the temperature value. Thetemperature drops the index increased, exposure time, each index change. Comparison of2℃and-20℃in rabbits exposed to blood enzyme changes can be found, the change degreeof each index of-20℃decreased, but with prolonged exposure time also showed a positiverelationship between the. Exposure to low temperature composite NaCN poisoning, moreobvious myocardial enzyme indexes of rabbit plasma than individual exposure to lowtemperature increase.60min after intoxication,-20℃CK-MB was1010.20±122.4, CK was1067.60±99.95, LDH was191.20±17.66(U/L), HBDH was126.40±19.54;-20℃exposureand sodium cyanide poisoning animal, the above indexes were1659.60±102.71,2632.80±244.63,395.40±38.61(U/L) and222±19.34, respectively is-20℃CK-MB1.64times, CK2.46times, LDH2.07times,1.76times HBDH. 2. The temperature drop, elevated liver function of rabbits with AST, ALT, ALPindicators, exposure time, the change of each index more intense. Exposure to lowtemperature composite NaCN poisoning, more obvious function in rabbits plasma liver thansingle exposure to low temperature increase. After the poisoning of60min,-20℃AST was47.20±4.30, ALT was53.20±5.63, ALP was165.80±14.45(U/L);-20℃exposure andsodium cyanide poisoning animal, the above indexes were273.40±14.90,67±6.81and277.40±17.64(U/L), respectively is-20℃AST5.79ALT times,1.26times,1.65timesALP.3. The temperature decrease, increase BU, CREA indicators of renal function in animal.Exposure time, the change of each index is more obvious. Exposure to low temperaturecomposite NaCN poisoning, more obvious in the plasma of rabbits renal function indexesBU, CREA, UA than single exposure to low temperature increase. After the poisoning of60min,-20℃BU was10.58±2.06, CREA was87.20±8.64, UA was10.40±0.77(U/L);-20℃exposure and sodium cyanide poisoning animal, the above indexes were11.68±1.24,103.20±10.91and132±9.17(U/L), respectively is-20℃BU1.10CREA times,1.18times,14.6times UA.4.4-DMAP after treatment, rabbit heart, liver, renal function indexes were lower than2℃without cyanide poisoning treated group, and the4-DMAP time is longer, the indexdecreased more significantly.60min after treatment, compared with the untreated2℃cyanide poisoning treated group of heart, liver, kidney function,4-DMAP after thetreatment of2℃cyanide poisoning group CK, CK-MB, LDH, α-HBDH, AST, ALT, ALP,BU, CREA were untreated2℃cyanide poisoning group42.96%,50.87%,56.94%,32.66%,44.64%,76.37%,49.72%,67.83%,76.67%.Conclusion：1. Low temperature exposure significantly affect myocardial enzymes in rabbit blood,liver function indices index and renal function. Exposure time is more long, the more effect.2. Low temperature exposure animal compound sodium cyanide poisoning, the rabbitheart, liver, kidney function changes more intense. Suggest that exposure to lowtemperature and sodium cyanide intoxication on rabbit heart, liver, kidney functioncombined effect. 3. Exposure to low temperature composite cyanide poisoning,4-DMAP can reduceexposure to low temperature injury effect on heart, liver, kidney function of the complex ofcyanide poisoning.