The Effect And Mechanism Of Polygalae Japonicae On The Airway Inflammation And Remodeling Of The Bronchial Asthmatic Mice

Objective:Study the therapeutic action and mechanism of the Polygala japonica to the Bronchial asthma mice models,what had got the airway inflammation and remodeling.Methods:First,prepared the male BALB/C mice60,whose weights are limited to the19-23g,were divided randomly into4groups:control group,bronchial asthma group, polygala japonica treated group and glucocorticoids positive control group.Every group has15mice.Second,except the control group,the others were sensitized with the mixed liquor on the1st and14th day,which is compounded with0.02mg OVA and0.001g Aluminum hydroxide gel in saline,the total volume is0.2ml for each mouse to do the intraperitoneal injection.Then,from the21st to23rd,the control group were challenged with the saline,simultaneously,the others were stimulated with an aerosol of the3%OVA in saline,each for30min.If the mice had been found abdominal muscle twitching,shortness of breath,restlessness,gatism,that were identified as the positive reaction.From17th to23rd, the polygala japonica treated group and glucocorticoids positive control group were treated with the appropriate dose of the drugs,which were given the gavage.When the last challenge was after24hours,all of the mice were executed,and the BALF,lung tissue were collected,what is according to the experimental design.The each group’s change of the cytokines’quantities in the BALF were determined with the ELISA.The each group’s count and change of the total cells and inflammatory cells in the BALF were observed with the Diff-Quick stain.The Morphological structure change of the bronchia and lung tissue were observed with the HE stain,PAS stain.The expression of NF-kB in the nucleu and cytolymph of the lung tissue were detected with the Western Blot.Result:(1)In the bronchial asthma group,the level of the interleukin-4,Interleu-kin-5,Interleukin-13in the BALF were significantly higher than the control group (P<0.05).(2) In the polygala japonica treated group and glucocorticoids positive control group,the above indicators were all significantly lower than the bronchial asthma group(P<0.05).(3) By the HE and PAS staining,compared with blank control group,in bronchial asthma model group we observed bronchus cavity contained large amounts of mucus plug,airway epithelial cell damaged, goblet cells proliferat-ed,basement membrane thickened,airway smooth muscle hyperplasia caused by muscular hypertrophy,and bronchial lumen contained a large number of inflammatory cells infiltrated around.In the polygala japonica treated group and glucocorticoids positive control group,the mucus plug,inflammatory cells infiltrated around and submucosa layer and mucosa hyperemia and edema are rarely seen.And the airway epithelial cell damaged,basement membrane thickened,goblet cells proliferat-ed,airway smooth muscle hyperplasia caused by muscular hypertrophy are much more reduced than the bronchial asthma model group.Conclusion:The Polygala japonica inhibits the airway inflammation and asthma attack,that can inhibit the release of Th2’s cytokines(IL-4,IL-5,IL-13) and the number of inflammatory cells in the BALF of the asthma mice,to reduce airway inflammation,like the airway mucus,vascular permeability increase, inflammatory cells infiltrate around.The Polygala japonica also inhibits the level of NF-kB expression to reduce the airway epithelial cell damaged,goblet cells prolifer-ated,basement membrane thickened,airway smooth muscle hyperplasia caused by muscular hypertrophy, relieve the airway remodeling.