Tanshinone ⅡA Inhibits The Oxidative Stress Reaction And Its Damage On Cultured Human Peritoneal Mesothelial Cells Induced By Peritoneal Dialysis Fluid

Objective:To investigate the effects of Tanshinone IIA on oxidative stress and its damage on cultured human peritoneal mesothelial cells (HPMCs) induced by peritoneal dialysis fluid (PDF).Methods:HPMCs cultured in vitro were used for our experiment. After being incubated with DMEM for24hours, the cells were divided into five groups (DMEM medium as control、PDFgroup、100umol/L Tanshinone II group%PDF containing50μMmol/L and100μMmol/L Tanshinone IIA as Tanshinone IIA1group and Tanshinone IIA2group. Each group was supplemented with equal volume of DMEM culture medium containing10%FCS. After48hours incubation the levels of MDA、SOD、GSH in the supernatant were collected for ROS generation and mitochondrial membrane potential (MMP) detection. Intracellular free calcium concentration was measured by immunofluorescence.Results:We found that PDF could promote the accumulation of MDA and superoxides in the supernatant, and reduce the levels of SOD、GSH at the same time. PDF also elevated the intracellular free calcium concentration,and decreased the mitochondrial membrane potential. All these changes of HPMCs induced by PDF were ameliorated by the addition of Tanshinone IIA into culture media.Conclusion: These findings demonstrate that Tanshinone IIA might protect the HPMCs from oxidative stress damage induced by PDF.