| Background and ObjectiveSecretion of matrix metalloproteinases may lead to extracellular matrix deposition, which has played an important role in tissue fibrosis in vivo, including peritoneal fibrosis. To investigate the effects of Tanshinone IIA on the secretion, mRNA and protein expression levels of matrix metalloproteinase-2and matrix metalloproteinase-9in human peritoneal mesothelial cells (HPMCs) induced by peritoneal dialysis fluid (PDF).MethodsHPMCs were cultured in vitro from HPMCs cell lines. All experiments were performed using cells between the first to third passages. Morphologic changes were detected through downward microscope during cell culture. The cultured cells were divided into five group:the control group, the Tanshinone IIA group, group with standard lactate-buffered4.25%glucose-based PDF, and two Tanshinone groups with standard lactate-buffered4.25%glucose-based PDF with different concentrations of Tanshinone ⅡA(50μuM and100μM). The cells were treated for72hours. The expressions of MMP-2、MMP-9mRNA and protein were measured by Real-Time PCR and Western Blot.ResultsAs compared with the control group, Exposure of HPMCs to PDS in vitro resulted in morphological change into a non-epitheloid shape.Both Real-Time PCR test and Western Blot showed PDF significantly upregulated MMP-2、MMP-9expressions of HPMCs in vitro as compared with the cells in control group. Tanshinone IIA significantly alleviated those changes induced by PDIf. And both of the two tests above showed that the expression levels of MMP-2、MMP-9mRNA and protein were downregulated in PDF containing Tanshinone IIA groups as compared with the PDF group. However, there is no significant difference between the two different concetrantions of Tanshinone groups.ConclusionHigh glucose-based PDF promotes the excessive J secretion and expression of MMP-2、-9in HPMCs. Tanshinone IIA may potentially counteract the change of HPMCs induced by PDF and delay peritoneal fibrosis. |
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