Association Between The NM23-H1Gene And Endometriosis

Background and purpose: Endometriosis is a common gynecological disease, which ischaracterised by chronic pelvic pain, dysmenorrhea.Nearly50%of patients suffer frominfertility.The pathogenesis is still unknown. Although endometriosis is a benign disease,it has some characteristics of malignancies, such as unrestricted growth, angiogenesis,reduced apoptotic cells, ectopic endometrial cell invasion, destruction of extracellularmatrix, local infiltration or remote transfer. Therefore, we hypothesize NM23-H1(NM23nucleoside diphosphate kinase1),a tumor metastasis related gene, may play animportant role in endometriosis. The experiment was designed to study the mRNAexpression of NM23-H1gene in endometriosis patients and normal controls in order toinvestigate the role of NM23-H1gene in the pathogenesis of endometriosis.Method: Using reverse transcription polymerase chain reaction (RT-PCR) and realtime reverse transcription polymerase chain reaction(real-time qPCR), we detect theexpression of NM23-H1gene and β-Actin gene in19endometriosis specimens and19normal controls. Then compare the the mRNA expression level of NM23-H1genebetween endometriosis and normal controls.，so as the results of real-time qPCRexperiment.Results:(1) The target bands of NM23-H1gene and β-Actin were found in allspecimens from the endometriosis and normal controls. The expression of NM23-H1mRNA is0.26±0.13in endometriosis group, and0.78±0.33in normal controls. ThemRNA expression levels of NM23-H1gene in endometriosis group are lower than thoseof normal tissues (P<0.05).(2)The NM23-H1mRNA expression in19endometriosis specimens and19normal tissues are0.65±0.51and1.32±1.09, respectively. ThemRNA expression levels of NM23-H1gene in endometriosis group are lower than thoseof normal tissues (P<0.05).Conclusion: The mRNA expression levels of NM23-H1gene in endometriosis tissuesare significantly lower than those of normal controls. The finding suggests thatNM23-H1gene may play a role in the pathogenesis of endometriosis. Furtherinvestigations on the exact pathogenesis of NM23-H1gene in endometriosis are neededto reveal the pathogenesis and find new strategies for clinical therapy and prognosis.