Experimental Research Of The Way To Establish The Chronic Heart Failure Model Of Pigs And The Effect Of Two Kinds Of Counterpulsation Devices

Part I Chronic heart failure model of pig joint establishmentof myocardial ischemia and myocardial toxicity of drugsObjective By porcine coronary artery ligation and continuous intravenous infusionof adriamycin to verify the effect of the establishment of animal model of chronic heartfailure.Method Randomly selected12healthy adult Panama miniature pig as the object ofstudy, body weight of45.6±2.1kg, both male and female. Ultrasonic Heartbeat graphto check heart function of each pig, left chestimplantation into varioushemodynamic device, ligation of the left anterior descending and (or)circumflex branches to establish acute heart failure model. The animals are feeded threemonths, during this period the intravenous injection of adriamycin in once a week.Three months later, another echocardiogram to check heart function of pigs each, andimplantable hemodynamic system. with the median sternotomy. Physiological recordercollected hemodynamic parameters which are before and after ligation of coronary andafter three months. Comparison of hemodynamic parameters changes before and afterthe experiment, used to evaluate the effect of the method to build the model of chronicfailure. Result Successfully established12animal model of acute myocardial ischemia.Ligation of the left anterior descending and circumflex branches (or), cardiacoutput (CO) and mean arterial pressure MAP) than before the experiment weredecreased by9.19%(P<0.01) and8.99%(P<0.01). Mean left atrialpressure (MLAP) and left ventricular end diastolic pressure (LVEDP) were increased by17.47%(P <0.01) and7.13%(P<0.01). Proved that the animal model of acutemyocardial ischemia of coronary arterybranch ligation can establish a stable animal.Intravenous adriamycin after ultrasonic Beckoning figure confirmed left ventricular enddiastolic diameter (LVEDD), left ventricular ejection fraction (LVEF) and COfrom40.57±1.57before the experiment,67.53±1.95and4.51±0.21become58.79±2.01,47.62±1.86and4.05±0.13, change rate is44%,25.52%and31.72%(P<0.01). Display of cardiac hemodynamic indexes, intravenous adriamycin, porcineCO,MAP, MLAP and LVEDP and other hemodynamic parameters are changed. CO isfrom4.51±0.21to4.05±0.13decline, of about10.20%(P<0.01). MAP alsodecreased about11.99%(P<0.01), from the previous90.35±2.31was reduced to79.52±3.97. But MLAP and LVEDP were significantly increased, respectively from23.12±2.19and55.05±4.33rise to27.93±1.18and61.09±3.34, increase rate is about20.80%and10.99%(P<0.01). Proven coronary artery ligation combined withintravenous adriamycin scheme can establish pig model of chronic heart failure.Conclusion Ligation of coronar yarteryanterior descending branch or circumflex can establish a stable acute heart failure.Experimental animals which are acute heart failure can tolerance long period andsmall dose of intravenous injection of adriamycin. The method which is intravenousinjection of adriamycin after ligation of coronary artery can establish the modelof chronic heart failure. Part II Comparison of the effect of IABP and PACD inchronic heart failure modelObjective Through the establishment of pig model of chronic heart failure, Wecomparedintraaortic balloon pump (IABP) and paraaortic counterpulsation device(PACD) secondary effects.Method Selects the first phase of the experiment has successfully establishedBAMA pig chronic heart failure model of10for the study, weight62.3±1.9kg, both themale and female. Middle into road into chest implant various blood flow dynamicsdevice, by test animal of iliac total artery implant a double cavity top with airbags andpressure sensor probe of IABP ball capsule catheter to chest down initiative pulse within,PACD is half spherical, was a diaphragm into blood capsule and airbags of loopauxiliary device, its blood capsule by single export of no flap artificial vascular matchesYu down initiative pulse. The balloon in the air ducts connected to the IABP console.Through the aortic pressure waveform trigger, synchronization counterpulsation supportcharge and discharge capacity replacement. Applied physiological recorder continuouslycollected two kinds of devices-aortic hemodynamics indexes before and after.Laboratory animals before and after the two devices-aortic hemodynamics indexes inorder to compare these two auxiliary effect of assistive devices.Result All10animals participate in and survive, pushing high-k and executed afterthe experiment was over. The IABP and PACD after secondary, ventricular afterload inexperimental animals significantly reduced, increased cardiac output, heart functionimproved left ventricular diastolic pressure increased, the haemodynamic indicatorshave improved significantly. The IABP and PACD after secondary and CO per cent and7.65%respectively, from4.05±±0.13per cent to4.36and0.22±0.18L/min (p<0.01).MLAP decreased by9.24%and18.47%, from27.93±1.18to25.35±1.10decline and22.77±1.06mmHg (P <0.01). Significant changes in LVEDP, decreased by7.69%and 13.88%, respectively, from61.09±3.34to56.39±3.32and52.61±4.51mmHg (P<0.05). Aortic systolic pressure (SAP) decline and4.73%from87.95±3.34mmHg±±3.5per cent and3.23(p<0.02). Aortic diastolic blood pressure (DAP) and9.39%dropped significantly from62.94±4.41mmHg±±and3.94to3.79per cent (p<0.02).LVEDP significantly reduced7.33%and five from74.93±3.58per cent±and4.37±4.68mmHg (p<0.02). Reflect the indirect indicators of coronary artery blood flow aorticflow (AABF) and pulmonary artery flow (PABF). AABF-PABF after twocounterpulsation devices supporting respectively increased significantly by41.27%(P＜0.05)，87.30%(P<0.05), rose0.63±0.89±0.26±0.31and1.18(L/min), mean aorticdiastolic pressure (MDAP)18.06%and35.38%respectively (p<0.05). When the MAPdown to60mmHg, intraaortic detection of two secondary effects after IABP support,CO,MAP and MADP reflect correct indexes of heart function, no significant statisticaldifference (P>0.05). The PACD after secondary, rose3.31±3indicators respectively6.22to79.53of3.84±L/min;60.17±±±5.37to58.43±6.02mmHg and6.79mmHg,16.01%,32.18%and28.96%respectively (P<0.05), a statistically significant differencebetween the effect of IABP and PACD (P<0.05).Conclusion After counter pulsation, SAP, DAP, LVDP were significantly reduced,two counter pulsation devices can significantly reduce animal chronic heart failure afterload; and the PACD effect is more obvious. After counter pulsation, CO, MAP, MDAPwere significantly increased, two kinds of counter pulsation devices can significantlyimprove heart function; and the PACD effect is more obvious. After counter pulsation,AABF, PABF, MDAP, PADP were significantly increased, two kinds of counterpulsation devices can significantly improve the coronary bloodflow, myocardial perfusion, myocardial oxygen consumption, and PACD has moreremarkable effect. The MAP below60mmHg level still can not believe that IABP canplay a supplementary effect, while PACD can play a supporting effect.