The Autologous Platelet-rich Plasma Was Activated With Bovine Thrombin On Rabbit Articular Cartilage Damage Repair

Objective: To validate whether it is ture that in vivo animal, Activationand not activated platelet-rich plasma in cartilage repair process are biologicaldifferences. the biological effects of60U and1000U bovine thrombincalcium chloride complexus as the activating agent on the activation onplatelet-rich plasma are also similar, which is confirmed in the Preliminarytest.Methods:48healthy New Zealand adult rabbits, weighing3.0kg-3.2kg,were randomly divided into four groups, each one has12. Double rabbitarticular cartilage defect model, each group of24knees, about3.5mm indiameter, depth of about1.5mm, Named60units of bovine thrombin calciumchloride complexes in group1,1000units of bovine thrombin, calciumchloride complexes in group2, not untired activated platelet-rich plasma inthe control group，sham group.10ml central arterial blood was tooken fromRabbit ears and secondary platelet-rich plasma prepared by the use ofsecondary platelet-rich plasma obtained by landesberg’s method. Group1platelet rich plasma activatied by calcium chloride complexes60units bovinethrombin, platelet-rich gel is made and replanted in modeling the defectarea,Group2platelet rich plasma activatied by calcium chloride complexes1000units bovine thrombin, platelet-rich gel is made and replanted intomodeling the defect area, Platelet-rich plasma is injection into modelingintra-articular directly in The control group. Sham group made model withoutdoing any processing.Animals were killed4at4weeks,8weeks,12weeks each group,Observation of general samples. Hematoxylin，safranin and fastgreen staining. analyze the repair area using Image-Pro Plus image softwareand O’Driscoll scoring criteria for histological score. Statistical analysis wasperformed using spss17.0.Results: Group1and group2had observed at4weeks,8weeks,12weeks when the results were similar, it’s better than the same period in thecontrol group’s result,Significantly better than the sham group during thesame period. There was is no different group1and group2modelingcompletely repaired cartilage defect area,Color, texture and normal cartilageat12weeks. Most of the control group modeling cartilage defect area to berepaired, the color a bit weak, less smooth, slightly soft texture.sham grouprepair obvious, visible scarring.Image software to analyze the results of therepair area:there was no significant difference in group1compared group2at4weeks8weeks12weeks. Group1and group2compared with thecontrol group no statistically significant difference at4weeks,8weeks.There were statistically significant group1, group2and control groups werecompared with it in the sham group at4weeks,8weeks,12weeks.O’Driscoll histological score: there was no significant difference in group1compared group2at4weeks8weeks12weeks. Group1and group2compared with the control group no statistically significant difference at4weeks,8weeks. There were statistically significant group1, group2andcontrol groups were compared with it in the sham group at4weeks,8weeks,12weeks.Conclusion: Platelet-rich plasma can promote cartilage defect to repair,Activated platelet-rich plasma is more significant effect than not activatedplatelet-rich plasma in promoting more significant effect on cartilage repair. The biological effectiveness of platelet rich plasma gel made out ofplatelet-rich plasma which is activated by60U or1000U of bovine thrombincalcium chloride compound is similar.