The Effects Of Sonovue Plus Ultrasound Radiation On Apoptosis Of Different Human Ovarian Cancer Cells Induced By Doxorubicin

Objective：To investigate the antitumor effect of SonoVue combinedwith DOX exposed to ultrasound radiation on human ovarian cancercells, and compare the differences between A2780s and SKOV3cellstreated with the same concentration of DOX and microbubble exposedto the same ultrasound radiation parameters.Methods：MTT was used to examine the growth inhibition of A2780scells treated with DOX for24h.The IC50of DOX was calculated. BothA2780s and SKOV3cells were randomly divided into4groups: controlgroup (group C)、 DOX group (group D)、ultrasound+DOX group(group U+D)、ultrasound+microbuble+DOX group (group U+M+D).The cell viability of A2780s and SKOV3cells in each group wereexamined with MTT after using the same ultrasonic irradiationparameters [Upon exposure to1MHz pulsed ultrasound beam (0.5W/cm2) for30s] and the same concentration of DOX (finalconcentration of1.0μg/ml) and10%(w/v) microbubble. The apoptoticmorphology of A2780s and SKOV3cells were observed with invertedmicroscope. And the apoptotic nuclei morphology of A2780s cells ineach group were further evaluated by Hoechst33258staining. Results： The IC50of DOX in A2780s cells was about1.0μg/mL. Wefound that, The survival rates of DOX group, ultrasound plus DOXgroup、 ultrasound plus microbuble combined with DOX group inA2780s cells were58%、54%、52%, In SKOV3cells, they were71%、60%、58%, respectively. compared to the control group (The controlsurvival rate is considered as100%), the proliferation of A2780s andSKOV3cells in all treatment groups was significantly inhibited.However, no significant differences (p>0.05) in survival were observedamong the treatment groups of both cells. Furthermore, the survival ofA2780s cells in each group was slightly but not statistically significantlower than that of the corresponding group of SKOV3cells（P>0.05）.The morphological changes of apoptosis of A2780s and SKOV3cellswere observed with inverted microscope. And the apoptotic lesions inthe nuclei of A2780s cells in each treatment group were further observedby Hoechst33258staining. We found that there is a tendency in thenumber of condensed nuclei (characteristic of apoptosis) in eachtreatment group, which is as follows: group C＜group D＜group(U+D)＜group (U+M+D). However, the difference was notstatistically significant (P>0.05).Conclusions： DOX can induce apoptosis of A2780s and SKOV3ovarian cancer cells, however, ultrasound and microbubble have nosignificant effect on the induction of apoptosis of DOX and the cell viability of both cells.