Effect Of Danlong Xingnao Fang On Angiogenic Factors In Rats Models Of Cerebral Focal Ischemia-reperfusion

Objective:To investigate the effect of the Danlong Xingnao Fang on on Angiogenic factors in Rats Models of Cerebral Focal Ischemia-reperfusionMethod:This experiment adopts the method of MCAO model with SD male rat ischemia-reperfusion injury,a successful builted survivals are divided into six groups at random:Danlong Xingnao Fang big dose group, Danlong Xingnao Fang middle dose group,Danlong Xingnao Fang small dose group,Nimodipine group, Model group and Sham group, each group of has eight, in addition to the Sham group, the rest of the group adopt MCAO method to build model,and recovered of2h reperfusion, Sham1only, external carotid artery ligation without ischemia reperfusion.12h later, determination of neurological function score. From the first day, gavage postoperative gastric drug intervention, once a day, seven days in a row, Model group and Sham group were given distilled water volume. seven days later, fRapidly decapitated, infarct size was measured by TTC staining, case morphology using paraffin-embedded sections were observed under a powerful microscope. By Mark cerebral ischemic tissue to determine the local CD34expression and microvessel endothelial cells density,Determination of VEGF, Flk-1, Ang-1, bFGF and other biochemical markers using immunohistochemical methods.Result:(1) neurological function score:model group compared with the sham group, neurological function score was significantly higher (P<0.01); reduced compared to neurological function score for each treatment group and the untreated group, with statistical significance (P<0.05); there were no Danlong refreshing party groups and nimodipine group differences (P>0.05); and no significant difference (P>0.05) between the groups Danlong refreshing side.(2) infarct size:the model group compared with the sham group, infarct size was significantly increased (P<0.01); reduce infarct area of each treatment group compared with the model group, with statistical significance (P<0.05); Danlong Xingnao Fang of large, medium dose group with the nimodipine group differences (P<0.05), there were no difference between Danlong Xingnao Fang low dose group and nimodipine group (P>0.05); Danlong Xingnao Fang, there was no significant difference (P>0.05) between the brain side of each group.(3) ischemic hippocampal region skin cells and microvascular density:the model group compared with the sham group, endothelial cells and microvessel density were increased (P<0.05); each treatment group compared with the model groups’microvascular endothelial cells density increases were statistically significant (P<0.05);there were no Danlong refreshing party groups and nimodipine group differences (P>0.05); and no significant difference (P>0.05) between the groups Danlong refreshing side.(4) ischemic hippocampus angiogenesis factor VEGF, Flk-1, Ang-1, bFGF protein expression:the model group compared with the sham group, VEGF, Flk-1, Ang-1, bFGF protein expression were increased (P<0.05); each treatment group compared with the model group increased expression was statistically significant (P<0.05); no Danlong refreshing side of each group and the nimodipine group compared to the significant difference (P>0.05); Dan Long There was no significant difference (P>0.05) between the groups refreshing side...