| Objective: The study was designed to evaluate whether the tendonhealing in the bone tunnel improved when the periosteal graft wrapped aroundthe tendon．To research the ability and mechanism of autogenous periosteumpromote he tendon healing in the bone tunnel. To provide the experiment datefor the filed of the tendon which wrapped by periosteum healing inside bonetunnel.Methods: In this study, we had chosen48New Zealand white rabbitswithin3-4months old. The rabbits were randomly divided into two groups:group A,and group B.All the rabbits feeding conditions were same. After therabbits were anesthetized by1%pentobarbital sodium, they were operated tobuild a model of ACL injury, and then the ACL reconstruction operations weredone on them. The right knee of each rabbit was defined to the experimentalknee. Each rabbit was given40000units gentamicin during operation toprevent infection. And in three days after operation gave each rabbit40000units gentamicin one time a day. They were applied with the regionaltreatment of surgery postoperative. In group A, the autogenous tendon graftwas wrapped with periosteum which was from the tibia. In che group B, theACL was reconstructed only with autogenous tendon. In each group,sixrabbits were killed randomly two weeks postoperative, six rabbits were killedrandomly four weeks postoperative, six rabbits were killed randomly sixweeks postoperative, six rabbits were killed randomly eight weekspostoperative. All the operated knee joints were dissected. Every6rabbits ineach group which were killed at the same time were divided to3groupsrandomly: group slice HE,group slice toluidine blue and group Electronmicroscopy. To evaluate whether the tendon healing in the bone tunnelimproved when the periosteal graft wrapped around the tendon． Results:1There were no postoperative complications such as infection happened.2All the rabbits knees have negative drawer test, it was in accordancewith the clinical requirements after the cruciate ligament reconstruction.3General observation In group A, two weeks postoperative, tendonand bone tunnel junction is not tight, loose connective tissue exist betweentendon and bone.4weeks postoperative tendon in bone tunnel was close tobone better than2weeks postoperative.6weeks postoperative tendon in bonetunnel was close to bone better than4weeks postoperative.8weekspostoperative tendon in bone tunnel was close to bone better than6weekspostoperative. The tendon healing in the bone tunnel is approved. In groupB,the tendon and bone tunnel junction is looser than group A at the sametime.4Histological observation In experimental group of group A, therewere lots of cells, which was in the majority with inflammatory cells andfibroblast, infiltrated in the edge of tendon and bone cracks. And there werelittle fibro-chondrocyte appeared. The reparative tissue was not obviousstained with HE staining and Toluidine blue staining. While as time goes on,reparative tissue filled in the tendon and bone cracks gradually increased.Fibro-chondrocyte and extracellular matrix increased gradually. Fibrillationand neovascularization could be seen, the HE staining, Toluidine blue stainingand Electron microscopy also show gradually which mean that thecomposition of cartilage increased gradually. While in group B, the healing oftendon and bone tunnel was slower than that of group A. Statistical analysisresults showed that the healing condition of group A was significantly betterthan group B (p <0.05).Conclusions:Periosteum has repair function for tendon and bone junction in the rabbitmodel in this study.It can induce and accelerate inflammatory reaction; prom-te the junction of cellular proliferation and extracellular matrix synthesis andinduce cartilaginous metaplasia and vascularization. |
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