| Objective:Diabetic nephropathy is one of the microvascular complications of diabetes,is also one of the important causes of ESRD. Thus, the significance of earlylesions to prevent and treat diabetic nephropathy significant.YiQiYangYinHuoXue’s prescription is mentor Professor Zhang Geng Liangsummarizing years of clinical experience, has played a role in the progressionof diabetic nephropathy prevention and protection of the kidney. Theexperimental diabetic rat model copied by intraperitoneal injection ofstreptozotocin, after giving the drug intervention. Recipe observed on bloodglucose in diabetic rats, renal function, blood lipids,24-hour urinary proteinexcretion, renal pathology morphology, kidney weight/body weight valuesand the impact of kidney tissue expression of endothelin-1, a preliminarystudy of the protective effect of the parties to the kidneys.Method:Selection of clean healthy male Wistar rats weighing about230-250g, were60, one week adaptive feeding normal diet, normal control group(N)consisting of12randomly selected, and the remaining48diabetic rat modelfor replication. According to55mg/kg body weight, a single intraperitonealinjection of1%of streptozotocin citrate buffer(buffer concentration of0.1mmol/L, pH4.5),1%of the normal control group received the samevolume of citrate single intraperitoneal injection buffer. After72hours, the tailvein of rats collected, measured blood glucose≥16.7mmol/L, while waterintake and urine output increased significantly in rats to determine thesuccessful model. The successful modeling of the48rats were randomlydivided into model group(M), YiQiYangYinHuoXue’s group(Chineseherbology group, H), medicine irbesartan group(western medicine group, W), YiQiYangYinHuoXue unite irbesartan group(combination group, H+W),each group of12. Building a week after the success, giving medicine dosagegroups according to10times the dose of adult mice, according to the doseadministered. Yiqiyangyinhuoxue’s group were given the party daily10ml/kg(equivalent to crude drug32.5g/kg) of body weight orally, irbesartan groupwere given irbesartan tablets suspension daily10ml/kg (equivalent to originaldrug2.5mg/ml) weight orally once a day. Combined group daily morningdose of medicine given equal gavage, daily afternoon given the same dose ofirbesartan gavage. Normal control group and model group were given an equalvolume of saline daily. Using metabolic cages to collect24-hour urine,measuring24-hour urinary albumin excretion rate. Administration of8weekends, fasted12hours,2%sodium pentobarbital anesthetized byintraperitoneal injection of20mg/kg body weight, take femoral artery, bloodglucose rats, renal function, blood lipids, renal production of specimens takenfor light microscopy morphological changes of renal pathological andimmunohistochemical determination of the expression of renal endothelin-1.Results:1GeneralNormal control group’s rats eating, drinking and urine were regular. Weighthad significant growth, color luster, good mental state, activities freely. Rats inmodel group were more drink, polyphagia, polyuria, emaciation, poor mentalstate, color yellow, decreased activity. Part of the rats with the progress of theexperiment, appear a series of diabetes complications, cataract, infection, limbedema, etc. Model group, irbesartan group,YiQiYangYinHuoXue’s group, thejoint group eventually death number of rats respectively1,1,1,1, improperanalysis of the main cause of death is to fill the stomach, or bite each other anddie.2Blood sugar changeModel group, YiQiYangYinHuoXue’s group, irbesartan group, joint groupblood sugar of rats were significantly higher than that of normal group, thereare significant differences(P﹤0.05); YiQiYangYinHuoXue’s group and combined group have certain hypoglycemic effect, blood glucose decreasedobviously compared with model group, there are significant differences(P﹤0.05); irbesartan group no significant hypoglycemic effect compared withmodel group, there was no significant difference（P﹥0.05).3Plasma endothelin-1and24h urinary endothelin-1changesModel group, YiQiYangYinHuoXue’s group, irbesartan group, joint grouprat plasma endothelin-1was significantly higher than that of normal group,there was significant difference(P﹤0.05); YiQiYangYinHuoXue’s group andjoint group can decrease plasma endothelin-1, compared with the model, therewas significant difference(P﹤0.05); there was no significant difference inirbesartan group and model group（P﹥0.05).Model group, YiQiYangYinHuoXue’s group, irbesartan group, joint grouprat urinary endothelin-1was significantly higher than that of normal group,there was significant difference(P﹤0.05); YiQiYangYinHuoXue’s group andjoint group can decrease urinary endothelin-1, compared with the model, therewas significant difference(P﹤0.05); there was no significant difference inirbesartan group and model group（P﹥0.05).4Kidney hypertrophy index changesModel group, YiQiYangYinHuoXue’s group, irbesartan group, jointgroup rat kidney hypertrophy index were higher than in normal group, there are significant differences (P﹤0.05);Compared with model group, three of the treatment group rats kidney hypertrophy index decreased, with significant difference(P﹤0.05);Compared with irbesartan group and irbesartan group, kidney hypertrophy index joint group group rats decreased significantly(P﹤0.05).5Renal function and24hours urinary albumin excretion rate of changeModel group, YiQiYangYinHuoXue’s group, irbesartan group, joint group rats of urea nitrogen, creatinine, significantly higher than the normalgroup, there are significant differences(P﹤0.05); three treatment groupcompared with model group, the rats of urea nitrogen, creatinine are lower, there are significant differences(P﹤0.05); Compared with irbesartan group and irbesartan group, urea nitrogen, creatinine joint group group rats decreased significantly(P﹤0.05).Model group of24hours urinary albumin excretion rate, increasedsignificantly compared with normal control group, there are significantdifferences(P﹤0.05); Three treatment group of24hours urinary albuminexcretion rate of rats compared with model group, accidentally significantdifference(P﹤0.05); Compared with irbesartan group and irbesartan group,24hours urinary albumin excretion rate joint group group rats decreasedsignificantly(P﹤0.05).6Blood lipid changeCompared with normal control group, model group, YiQiYangYinHuoXue’sgroup, irbesartan group, joint group rats blood lipid increased significantly;YiQiYangYinHuoXue’s group and joint group have lipid-lowering effect,compared with model group, there are significant differences(P﹤0.05); Butirbesartan group compared with model group there was no significantdifference（P﹥0.05).7The influence of kidney tissue pathological morphologyNormal control group glomerular structure are clear and complete not seenglomerular volume increase, not seen glomerular capillary basementmembrane, mesangial matrix changes; Model group kidney structure disorder,glomerular volume are increased, the increase in the number of nucleiwidened mesangial area. Three treatment group compared with model group,have different degrees of ease, with joint group is most obvious.8The influence of kidney endothelin-1expressionET-1chemical staining showed immune histochemical method, normalgroup of renal glomerular and renal tubular epithelial cells have very weakexpression. Model group kidney glomerular endothelial cells and renal tubularepithelial cells showed strong positive expression of ET-1, significantly higherthan the normal group. Three treatment group compared with model group,expression is not drop, compared with single drug groups （P﹤0.05),especially the joint group decreased more obvious. Conclusion:1YiQiYangYinHuoXue’s prescription can reduce the urine proteinexcretion of diabetic rats, at the same time reduce the value of urea nitrogen,creatinine, delay the development of diabetic nephropathy rats, so asto protectthe kidney.2YiQiYangYinHuoXue’s prescription has hypoglycemic effect, by lowering the blood sugar to protect the kidney function.3YiQiYangYinHuoXue’s prescription can inhibit the expression of diabeticrats kidney ET-1, decrease the content of-1in plasma, urine endothelial,protect the structure and function of the kidney, provide new way fortraditional Chinese medicine treatment of diabetic nephropathy. |
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