Effect Of Human Umbilical Cord Mesenchymal Stem Cells On Hepatoma Cell Line In Vitro And In Vivo

Mesenchymal stem cells (MSCs) were initially identified as a heterogeneous population of stromal cells in the bone marrow that support hematopoietic stem cells. Although the infiltration of mesenchymal stem cells into different tumors are widely recognized in animal models, the question whether these MSCs have a positive or negative effect on disease progession remains unanswered. The aim of this study is to investigate whether MSCs affect hepatoma cell line HepG2growth invitro and in vivo.MSCs were isolated from human umbilical cords and the expanded cells were phenotyped by flow cytometry. Here we studied for the first time the association of supernate of human umbilical cords MSCs and the hepatoma cell line HepG2growth. Human umbilical cords derived MSCs supernate were uesed to stimulate HepG2, and this was followed by flow cytometry to examine cell cycle and apoptosis and CCk-8to determine the cell proliferation. Then, MSCs and hepatoma cell line HepG2were transplanted into NOD/SCID mice to observe the growth of tumor.The data showed that when co-culture with MSCs supernate, HepG2cell proliferation and apoptosis decreased and majority of HepG2cells were arrested in G2phase. Animal transplantaion studies showed that coengraftment of hepatoma cell line HepG2with MSCs resulted in substantially smaller tumors compared with paired engraftment of HepG2alone (n=6, P<0.01). Thus, MSCs may represent a new avenue for liver tumor therapeutic intervention.