Effect Of Curing-injury Cataplasma On Pain Behavior And The Expression Of The Nav1.7in Model Rats With Formaldehyde-induced Inflammatory Pain

Objective:To observe the analgesia of Curing-injury Cataplasma and discuss the PGE2in muscle tissue and Nav1.7expression in dorsal root ganglion in the Model Rats with Formaldehyde-induced Inflammatory Pain.Methods:36Sprague-Dawley rats were divided into three groups:1) blank control group (n=12),2) model control group (n=12),3) treatment group (n=12). The blank control group without any treatment, the model control group were injected0.1ml saline formalin, the treatment group were paste Curing-injury Cataplasma and after24h, they were injected0.1ml saline formalin,then Observation rat pain behavior reaction and use Dubuisson score method to record the comparison, meanwhile, the muscle of the rats and L3-6dorsal root ganglion were collected from36rats. The expression of PEG2was detected by Elisa and Navl.7was detected by real—time quantitative PCR and Western blot.Results:1. The blank control group of rats without pain behavioral responses, and the Pain reaction integral in the model control group higher than treatment group(P<0.01).2. Elisa results:PGE2expression in the model control group higher than the blank control group and treatment group(P<0.05), also, the treatment group is higher than the blank control group, but Within the group, they have no difference.3. RT-PCR results:Nav1.7mRNA expression in the model control group higher than the blank control group and treatment group(P<0.01), also, the treatment group is higher than the blank control group, but Within the group, they have no difference.4. Western blotting results:Nav1.7protein expression in the model control group higher than the blank control group and treatment group(P <0.01), also, the treatment group is higher than the blank control group, but Within the group, they have no difference.1. Curing-injury Cataplasma can alleviate inflammatory pain response in rats, and have certain analgesia effect.2. Curing-injury Cataplasma can reduce the release of PGE2.3. Each group rat dorsal root ganglia were stably expressed in Nav1.7, and formalin model of inflammatory pain was significantly increased expression of Nav1.7.4. The curing-injury cataplasma can affect formalin model of inflammatory pain in the rat dorsal root ganglion Nav1.7expression and can relieve inflammatory pain.