The Histomorphological Observation And Subcutaneous Embedded Experiment Research Of Autologous Concentrated Growth Factors Fibrin Gel And Membrane

Objective:The autologous Concentrated Growth Factors(CGF) fibrin gel is a extracfrom blood containing lots of fibrins and growth factors. It can be used in thehard and soft tissue defect. This study is to observe histomorphologicalstructure of CGF by using optical microscope and scanning electronmicroscopy, and investigate the characteristics of histomorphological structureto provide theoretical foundation for the clinical application of CGF gel andCGF membrane. And investigate CGF gel and CGF membrane as a scaffoldmaterial through subcutaneous embedded experiment to provide theoreticalfoundation for the clinical application of CGF.Methods:The first experiment is to observe the tissue structures of CGF gel andmembrane. Venous blood was obtained from6normal volunteers, and wascentrifuged in9ml tube. The prepared CGF was divided into group CGF geland CGF membrane. HE staining, Wright staining and Scanning ElectronMicroscopy were used to observe the structure of them.The second experiment is subcutaneous embedded test of CGF gel andCGF membrane. The venous blood was collected from New Zealand whiterabbits. The prepared CGF was separated into CGF gel and CGF membrane.The two kinds CGF were embedded in subcutaneous of hind legs of rabbits.These rabbits were executed on postoperative1,2and3weeks. The samplesof CGF and surrouding tissue were obtained and were stained with HE.Results:HE staining showed that mass red fibrins with a loosen networks wereobserved on the top of CGF gel while mass leukocytes were observed at the bottom of CGF gel. In addition, mass red fibrins with a concentrated networkswere observed on the top of CGF membrane while mass leukocytes wereobserved at the bottom of CGF membrane. Wright staining showed that bluishviolet loosen fibrins networks with few bluish violet platelets were observedupside and in the middle of CGF gel. However, the quantity of platelets ofupside CGF gel was less than that of middle of CGF gel. Mass bluish violetleukocytes and platelets were observed at the bottom of CGF gel and irregularplatelets were connected with fibrins networks. The integral structure of CGFmembrane is similar as that of CGF gel. However, CGF membrane iscomposed of more denser fibrins networks and platelets. SEM showed thatconcentrated fibrins networks with mass platelets were observed in upside ofthe CGF gel while loosen fibrins networks with mass erythrocyte, leukocyteand platelets were observed at the bottom of the CGF gel.After one week, both CGF gel and CGF membrane were smaller thanbefore and were not connected with tissues. The edge of the CGF was brokeninto pieces with a few of fibroblasts. After2weeks, most of CGF wasabsorbed and CGF which was connected with tissue remained a quarter of theoriginal volume. The surounding connective tissues were mixed with CGF andinflammatory cells had not been found in the surounding connective tissues.After3weeks, CGF were compeletely absorbed and replaced by newconnective tissues.Conclusion:1. Both CGF gel and CGF membrane were made up of benignthree-dimensional network structures. Fibrin networks in CGF membranewere denser than that in CGF gel.2. Both CGF gel and CGF membrane contain sporadic platelets mass,and their possible function is to release some growth factors. 3. Both CGF gel and CGF membrane can be used as a filling material, torepair deformities and collapse of maxillofacial soft tissue.